Experiment: MDC_bicarb with butyrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_000260 and TX73_000265 are separated by 57 nucleotides TX73_000265 and TX73_000270 are separated by 275 nucleotides TX73_000270 and TX73_000275 are separated by 602 nucleotides TX73_000275 and TX73_000280 are separated by 50 nucleotides
TX73_000260: TX73_000260 - RNA polymerase factor sigma-54, at 56,042 to 57,688
_000260
TX73_000265: TX73_000265 - ribosome-associated translation inhibitor RaiA, at 57,746 to 58,351
_000265
TX73_000270: TX73_000270 - PTS IIA-like nitrogen regulatory protein PtsN, at 58,627 to 59,088
_000270
TX73_000275: TX73_000275 - DUF1150 domain-containing protein, at 59,691 to 59,957
_000275
TX73_000280: TX73_000280 - Hsp20 family protein, at 60,008 to 60,433
_000280
Position (kb) 58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 57.636 kb on - strand at 57.646 kb on + strand at 57.649 kb on - strand at 57.654 kb on - strand at 57.662 kb on - strand at 57.677 kb on + strand at 57.677 kb on + strand at 57.685 kb on - strand at 57.685 kb on - strand at 57.685 kb on - strand at 57.691 kb on + strand at 57.701 kb on + strand at 57.701 kb on + strand at 57.701 kb on + strand at 57.704 kb on - strand at 57.704 kb on - strand at 57.704 kb on - strand at 57.704 kb on - strand at 57.706 kb on + strand at 57.709 kb on - strand at 57.709 kb on - strand at 57.728 kb on - strand at 57.743 kb on + strand at 57.751 kb on - strand at 57.751 kb on - strand at 57.751 kb on - strand at 57.751 kb on - strand at 57.783 kb on + strand at 57.799 kb on - strand at 57.816 kb on + strand, within TX73_000265 at 57.832 kb on - strand, within TX73_000265 at 57.847 kb on + strand, within TX73_000265 at 57.847 kb on + strand, within TX73_000265 at 57.870 kb on - strand, within TX73_000265 at 57.871 kb on + strand, within TX73_000265 at 57.873 kb on + strand, within TX73_000265 at 57.890 kb on - strand, within TX73_000265 at 57.905 kb on + strand, within TX73_000265 at 57.938 kb on - strand, within TX73_000265 at 57.946 kb on + strand, within TX73_000265 at 57.967 kb on - strand, within TX73_000265 at 57.974 kb on + strand, within TX73_000265 at 57.981 kb on + strand, within TX73_000265 at 57.982 kb on - strand, within TX73_000265 at 57.990 kb on + strand, within TX73_000265 at 57.991 kb on - strand, within TX73_000265 at 58.022 kb on - strand, within TX73_000265 at 58.023 kb on + strand, within TX73_000265 at 58.031 kb on - strand, within TX73_000265 at 58.054 kb on + strand, within TX73_000265 at 58.055 kb on + strand, within TX73_000265 at 58.060 kb on + strand, within TX73_000265 at 58.086 kb on - strand, within TX73_000265 at 58.086 kb on - strand, within TX73_000265 at 58.156 kb on - strand, within TX73_000265 at 58.174 kb on + strand, within TX73_000265 at 58.174 kb on + strand, within TX73_000265 at 58.195 kb on - strand, within TX73_000265 at 58.204 kb on + strand, within TX73_000265 at 58.210 kb on + strand, within TX73_000265 at 58.214 kb on - strand, within TX73_000265 at 58.252 kb on - strand, within TX73_000265 at 58.266 kb on + strand, within TX73_000265 at 58.284 kb on + strand, within TX73_000265 at 58.331 kb on + strand at 58.339 kb on - strand at 58.339 kb on - strand at 58.339 kb on - strand at 58.339 kb on - strand at 58.339 kb on - strand at 58.358 kb on - strand at 58.372 kb on - strand at 58.381 kb on + strand at 58.399 kb on + strand at 58.407 kb on - strand at 58.414 kb on - strand at 58.423 kb on + strand at 58.424 kb on + strand at 58.431 kb on - strand at 58.431 kb on - strand at 58.432 kb on - strand at 58.551 kb on + strand at 58.559 kb on - strand at 58.564 kb on - strand at 58.577 kb on - strand at 58.582 kb on - strand at 58.604 kb on + strand at 58.612 kb on + strand at 58.693 kb on - strand, within TX73_000270 at 58.721 kb on + strand, within TX73_000270 at 58.729 kb on - strand, within TX73_000270 at 58.736 kb on + strand, within TX73_000270 at 58.743 kb on - strand, within TX73_000270 at 58.754 kb on + strand, within TX73_000270 at 58.762 kb on + strand, within TX73_000270 at 58.802 kb on + strand, within TX73_000270 at 58.802 kb on + strand, within TX73_000270 at 58.802 kb on + strand, within TX73_000270 at 58.802 kb on + strand, within TX73_000270 at 58.802 kb on + strand, within TX73_000270 at 58.810 kb on - strand, within TX73_000270 at 58.810 kb on - strand, within TX73_000270 at 58.841 kb on - strand, within TX73_000270 at 58.856 kb on + strand, within TX73_000270 at 58.868 kb on - strand, within TX73_000270 at 58.961 kb on + strand, within TX73_000270 at 58.962 kb on + strand, within TX73_000270 at 58.962 kb on + strand, within TX73_000270 at 58.962 kb on + strand, within TX73_000270 at 58.962 kb on + strand, within TX73_000270 at 58.962 kb on + strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 58.970 kb on - strand, within TX73_000270 at 59.008 kb on - strand, within TX73_000270 at 59.064 kb on - strand at 59.067 kb on + strand at 59.091 kb on + strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.091 kb on - strand at 59.103 kb on + strand at 59.111 kb on - strand at 59.113 kb on + strand at 59.136 kb on - strand at 59.156 kb on + strand at 59.157 kb on + strand at 59.185 kb on - strand at 59.203 kb on + strand at 59.211 kb on - strand at 59.225 kb on + strand at 59.234 kb on + strand at 59.237 kb on + strand at 59.239 kb on + strand at 59.252 kb on - strand at 59.252 kb on - strand at 59.269 kb on + strand at 59.269 kb on + strand at 59.277 kb on - strand at 59.285 kb on - strand at 59.285 kb on - strand at 59.307 kb on + strand at 59.327 kb on + strand at 59.344 kb on - strand at 59.375 kb on + strand at 59.377 kb on + strand at 59.378 kb on - strand at 59.401 kb on + strand at 59.423 kb on - strand at 59.429 kb on + strand at 59.429 kb on + strand at 59.429 kb on + strand at 59.434 kb on + strand at 59.435 kb on + strand at 59.435 kb on + strand at 59.437 kb on - strand at 59.443 kb on - strand at 59.465 kb on + strand at 59.506 kb on + strand at 59.514 kb on - strand at 59.515 kb on - strand at 59.537 kb on - strand at 59.541 kb on + strand at 59.633 kb on - strand at 59.695 kb on - strand at 59.720 kb on + strand, within TX73_000275 at 59.721 kb on + strand, within TX73_000275 at 59.721 kb on + strand, within TX73_000275 at 59.722 kb on - strand, within TX73_000275 at 59.782 kb on + strand, within TX73_000275 at 59.790 kb on - strand, within TX73_000275 at 59.790 kb on - strand, within TX73_000275 at 59.790 kb on - strand, within TX73_000275 at 59.809 kb on - strand, within TX73_000275 at 59.809 kb on - strand, within TX73_000275 at 59.857 kb on + strand, within TX73_000275 at 59.875 kb on - strand, within TX73_000275 at 59.885 kb on - strand, within TX73_000275 at 59.888 kb on - strand, within TX73_000275 at 59.892 kb on + strand, within TX73_000275 at 59.912 kb on + strand, within TX73_000275 at 59.917 kb on - strand, within TX73_000275 at 59.937 kb on + strand at 59.941 kb on + strand at 59.944 kb on - strand at 59.966 kb on - strand at 59.967 kb on - strand at 59.968 kb on + strand at 59.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MDC_bicarb with butyrate remove 57,636 - +1.0 57,646 + +0.6 57,649 - -0.1 57,654 - -1.8 57,662 - +0.7 57,677 + +0.9 57,677 + +1.4 57,685 - +1.1 57,685 - +1.5 57,685 - +1.4 57,691 + -2.7 57,701 + -0.2 57,701 + +0.1 57,701 + +1.3 57,704 - -0.2 57,704 - -0.8 57,704 - -1.0 57,704 - +0.2 57,706 + -0.2 57,709 - -0.2 57,709 - -1.0 57,728 - +0.0 57,743 + -0.7 57,751 - +0.3 57,751 - +0.0 57,751 - +0.3 57,751 - -0.5 57,783 + -0.7 57,799 - -0.3 57,816 + TX73_000265 0.12 +0.4 57,832 - TX73_000265 0.14 -0.1 57,847 + TX73_000265 0.17 -0.0 57,847 + TX73_000265 0.17 -0.6 57,870 - TX73_000265 0.20 -0.2 57,871 + TX73_000265 0.21 +0.0 57,873 + TX73_000265 0.21 +0.2 57,890 - TX73_000265 0.24 -0.6 57,905 + TX73_000265 0.26 -1.2 57,938 - TX73_000265 0.32 -0.2 57,946 + TX73_000265 0.33 +0.1 57,967 - TX73_000265 0.36 -0.3 57,974 + TX73_000265 0.38 -0.5 57,981 + TX73_000265 0.39 -0.3 57,982 - TX73_000265 0.39 -0.8 57,990 + TX73_000265 0.40 +0.1 57,991 - TX73_000265 0.40 -0.3 58,022 - TX73_000265 0.46 +1.1 58,023 + TX73_000265 0.46 +0.5 58,031 - TX73_000265 0.47 -0.8 58,054 + TX73_000265 0.51 -2.0 58,055 + TX73_000265 0.51 -1.2 58,060 + TX73_000265 0.52 -0.3 58,086 - TX73_000265 0.56 +0.0 58,086 - TX73_000265 0.56 +0.0 58,156 - TX73_000265 0.68 +0.8 58,174 + TX73_000265 0.71 +0.0 58,174 + TX73_000265 0.71 -0.1 58,195 - TX73_000265 0.74 -0.5 58,204 + TX73_000265 0.76 -0.4 58,210 + TX73_000265 0.77 -0.5 58,214 - TX73_000265 0.77 -0.3 58,252 - TX73_000265 0.83 -0.7 58,266 + TX73_000265 0.86 +0.1 58,284 + TX73_000265 0.89 -1.1 58,331 + -0.5 58,339 - -0.2 58,339 - -0.5 58,339 - -1.8 58,339 - -0.4 58,339 - +0.3 58,358 - +0.4 58,372 - +0.4 58,381 + -2.1 58,399 + +0.6 58,407 - +0.4 58,414 - -1.3 58,423 + -0.4 58,424 + +0.2 58,431 - -0.4 58,431 - -0.0 58,432 - -1.0 58,551 + -0.2 58,559 - -0.4 58,564 - +0.1 58,577 - +0.0 58,582 - -0.4 58,604 + -0.8 58,612 + -0.3 58,693 - TX73_000270 0.14 -1.2 58,721 + TX73_000270 0.20 -1.3 58,729 - TX73_000270 0.22 +2.2 58,736 + TX73_000270 0.24 -0.5 58,743 - TX73_000270 0.25 -1.7 58,754 + TX73_000270 0.27 -2.0 58,762 + TX73_000270 0.29 +0.6 58,802 + TX73_000270 0.38 -0.8 58,802 + TX73_000270 0.38 -0.1 58,802 + TX73_000270 0.38 -1.5 58,802 + TX73_000270 0.38 -0.3 58,802 + TX73_000270 0.38 -0.9 58,810 - TX73_000270 0.40 -1.8 58,810 - TX73_000270 0.40 -0.1 58,841 - TX73_000270 0.46 -0.4 58,856 + TX73_000270 0.50 -2.3 58,868 - TX73_000270 0.52 -1.0 58,961 + TX73_000270 0.72 +0.1 58,962 + TX73_000270 0.73 -1.5 58,962 + TX73_000270 0.73 -0.9 58,962 + TX73_000270 0.73 -1.4 58,962 + TX73_000270 0.73 -0.8 58,962 + TX73_000270 0.73 -1.7 58,970 - TX73_000270 0.74 +1.4 58,970 - TX73_000270 0.74 +0.3 58,970 - TX73_000270 0.74 -2.8 58,970 - TX73_000270 0.74 -2.6 58,970 - TX73_000270 0.74 -2.3 58,970 - TX73_000270 0.74 -1.5 59,008 - TX73_000270 0.82 +0.2 59,064 - +0.1 59,067 + -0.3 59,091 + -0.2 59,091 - +0.0 59,091 - -0.2 59,091 - -1.0 59,091 - -0.4 59,091 - +0.1 59,091 - -0.7 59,091 - -0.6 59,103 + +0.3 59,111 - +0.2 59,113 + +1.0 59,136 - +2.4 59,156 + -1.2 59,157 + -0.6 59,185 - +1.2 59,203 + +0.9 59,211 - -0.0 59,225 + +0.3 59,234 + +0.4 59,237 + -0.3 59,239 + +0.3 59,252 - +0.6 59,252 - +0.9 59,269 + +0.6 59,269 + +0.3 59,277 - +2.6 59,285 - -0.5 59,285 - -0.3 59,307 + +0.0 59,327 + -0.6 59,344 - +1.4 59,375 + +0.5 59,377 + -0.9 59,378 - -0.2 59,401 + -0.4 59,423 - +0.9 59,429 + +0.2 59,429 + -0.3 59,429 + -0.5 59,434 + +0.0 59,435 + +1.1 59,435 + +2.0 59,437 - +0.0 59,443 - -1.2 59,465 + +0.1 59,506 + -1.0 59,514 - +0.7 59,515 - +0.7 59,537 - -0.4 59,541 + -0.2 59,633 - +0.5 59,695 - +0.2 59,720 + TX73_000275 0.11 +0.1 59,721 + TX73_000275 0.11 +0.3 59,721 + TX73_000275 0.11 -2.2 59,722 - TX73_000275 0.12 +0.1 59,782 + TX73_000275 0.34 -0.9 59,790 - TX73_000275 0.37 +1.4 59,790 - TX73_000275 0.37 +0.1 59,790 - TX73_000275 0.37 -0.2 59,809 - TX73_000275 0.44 -0.3 59,809 - TX73_000275 0.44 +0.6 59,857 + TX73_000275 0.62 +0.0 59,875 - TX73_000275 0.69 +0.1 59,885 - TX73_000275 0.73 -0.2 59,888 - TX73_000275 0.74 -0.2 59,892 + TX73_000275 0.75 -0.1 59,912 + TX73_000275 0.83 -0.0 59,917 - TX73_000275 0.85 -0.2 59,937 + +0.4 59,941 + -0.1 59,944 - +1.4 59,966 - -0.2 59,967 - -0.1 59,968 + +0.8 59,969 + +1.5
Or see this region's nucleotide sequence