Experiment: NFM with pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_002165 and TX73_002170 are separated by 81 nucleotides TX73_002170 and TX73_002175 are separated by 103 nucleotides
TX73_002165: TX73_002165 - enoyl-CoA hydratase, at 458,633 to 459,445
_002165
TX73_002170: TX73_002170 - nuclear transport factor 2 family protein, at 459,527 to 460,027
_002170
TX73_002175: TX73_002175 - glutamine amidotransferase, at 460,131 to 460,928
_002175
Position (kb) 459
460
461 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 458.528 kb on + strand at 458.531 kb on - strand at 458.531 kb on - strand at 458.531 kb on - strand at 458.536 kb on + strand at 458.536 kb on + strand at 458.536 kb on - strand at 458.541 kb on - strand at 458.583 kb on + strand at 458.612 kb on + strand at 458.626 kb on - strand at 458.626 kb on - strand at 458.626 kb on - strand at 458.626 kb on - strand at 458.626 kb on - strand at 458.640 kb on + strand at 458.648 kb on - strand at 458.648 kb on - strand at 458.685 kb on + strand at 458.686 kb on + strand at 458.686 kb on + strand at 458.687 kb on + strand at 458.693 kb on - strand at 458.693 kb on - strand at 458.694 kb on - strand at 458.705 kb on - strand at 458.738 kb on + strand, within TX73_002165 at 458.754 kb on + strand, within TX73_002165 at 458.769 kb on + strand, within TX73_002165 at 458.769 kb on + strand, within TX73_002165 at 458.777 kb on - strand, within TX73_002165 at 458.777 kb on - strand, within TX73_002165 at 458.777 kb on - strand, within TX73_002165 at 458.782 kb on - strand, within TX73_002165 at 458.784 kb on - strand, within TX73_002165 at 458.799 kb on - strand, within TX73_002165 at 458.837 kb on + strand, within TX73_002165 at 458.845 kb on - strand, within TX73_002165 at 458.846 kb on - strand, within TX73_002165 at 458.855 kb on + strand, within TX73_002165 at 458.865 kb on + strand, within TX73_002165 at 458.865 kb on + strand, within TX73_002165 at 458.868 kb on + strand, within TX73_002165 at 458.885 kb on - strand, within TX73_002165 at 458.887 kb on + strand, within TX73_002165 at 458.887 kb on + strand, within TX73_002165 at 458.887 kb on + strand, within TX73_002165 at 458.889 kb on + strand, within TX73_002165 at 458.895 kb on - strand, within TX73_002165 at 458.895 kb on - strand, within TX73_002165 at 458.895 kb on - strand, within TX73_002165 at 458.934 kb on + strand, within TX73_002165 at 458.940 kb on - strand, within TX73_002165 at 458.940 kb on - strand, within TX73_002165 at 458.946 kb on + strand, within TX73_002165 at 458.976 kb on + strand, within TX73_002165 at 458.984 kb on - strand, within TX73_002165 at 458.990 kb on - strand, within TX73_002165 at 458.995 kb on - strand, within TX73_002165 at 459.021 kb on - strand, within TX73_002165 at 459.024 kb on + strand, within TX73_002165 at 459.032 kb on - strand, within TX73_002165 at 459.032 kb on - strand, within TX73_002165 at 459.032 kb on - strand, within TX73_002165 at 459.034 kb on + strand, within TX73_002165 at 459.042 kb on - strand, within TX73_002165 at 459.042 kb on - strand, within TX73_002165 at 459.042 kb on - strand, within TX73_002165 at 459.060 kb on + strand, within TX73_002165 at 459.068 kb on - strand, within TX73_002165 at 459.068 kb on - strand, within TX73_002165 at 459.068 kb on - strand, within TX73_002165 at 459.085 kb on - strand, within TX73_002165 at 459.094 kb on + strand, within TX73_002165 at 459.094 kb on + strand, within TX73_002165 at 459.102 kb on + strand, within TX73_002165 at 459.110 kb on - strand, within TX73_002165 at 459.145 kb on + strand, within TX73_002165 at 459.217 kb on - strand, within TX73_002165 at 459.217 kb on - strand, within TX73_002165 at 459.217 kb on - strand, within TX73_002165 at 459.222 kb on - strand, within TX73_002165 at 459.238 kb on + strand, within TX73_002165 at 459.276 kb on + strand, within TX73_002165 at 459.367 kb on + strand at 459.394 kb on - strand at 459.427 kb on - strand at 459.443 kb on + strand at 459.477 kb on - strand at 459.479 kb on + strand at 459.484 kb on + strand at 459.484 kb on + strand at 459.492 kb on - strand at 459.543 kb on + strand at 459.543 kb on + strand at 459.543 kb on + strand at 459.543 kb on + strand at 459.543 kb on + strand at 459.543 kb on + strand at 459.551 kb on - strand at 459.551 kb on - strand at 459.551 kb on - strand at 459.551 kb on - strand at 459.551 kb on - strand at 459.576 kb on + strand at 459.576 kb on + strand at 459.620 kb on - strand, within TX73_002170 at 459.714 kb on - strand, within TX73_002170 at 459.723 kb on - strand, within TX73_002170 at 459.744 kb on + strand, within TX73_002170 at 459.752 kb on - strand, within TX73_002170 at 459.760 kb on + strand, within TX73_002170 at 459.761 kb on + strand, within TX73_002170 at 459.783 kb on + strand, within TX73_002170 at 459.789 kb on + strand, within TX73_002170 at 459.789 kb on + strand, within TX73_002170 at 459.791 kb on - strand, within TX73_002170 at 459.792 kb on - strand, within TX73_002170 at 459.955 kb on + strand, within TX73_002170 at 459.986 kb on + strand at 460.028 kb on + strand at 460.051 kb on - strand at 460.056 kb on - strand at 460.093 kb on + strand at 460.095 kb on + strand at 460.136 kb on - strand at 460.136 kb on - strand at 460.230 kb on + strand, within TX73_002175 at 460.230 kb on + strand, within TX73_002175 at 460.238 kb on - strand, within TX73_002175 at 460.306 kb on - strand, within TX73_002175 at 460.345 kb on - strand, within TX73_002175 at 460.349 kb on + strand, within TX73_002175 at 460.364 kb on - strand, within TX73_002175 at 460.370 kb on - strand, within TX73_002175 at 460.504 kb on - strand, within TX73_002175 at 460.529 kb on + strand, within TX73_002175 at 460.628 kb on - strand, within TX73_002175 at 460.662 kb on + strand, within TX73_002175 at 460.663 kb on + strand, within TX73_002175 at 460.699 kb on + strand, within TX73_002175 at 460.706 kb on - strand, within TX73_002175 at 460.707 kb on - strand, within TX73_002175 at 460.709 kb on - strand, within TX73_002175 at 460.775 kb on + strand, within TX73_002175 at 460.796 kb on + strand, within TX73_002175 at 460.804 kb on - strand, within TX73_002175 at 460.929 kb on + strand at 460.929 kb on + strand at 460.932 kb on - strand at 460.932 kb on - strand at 460.934 kb on + strand at 460.934 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.939 kb on + strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.947 kb on - strand at 460.962 kb on - strand at 460.970 kb on + strand at 461.005 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NFM with pyruvate remove 458,528 + +0.2 458,531 - +0.9 458,531 - +0.3 458,531 - +1.7 458,536 + +0.6 458,536 + +0.9 458,536 - +0.4 458,541 - -0.5 458,583 + +1.3 458,612 + -0.1 458,626 - -2.3 458,626 - -0.1 458,626 - -0.7 458,626 - +1.2 458,626 - +1.0 458,640 + -0.9 458,648 - -0.4 458,648 - +1.2 458,685 + +1.0 458,686 + -1.4 458,686 + -0.1 458,687 + -0.0 458,693 - +1.7 458,693 - +0.3 458,694 - +0.8 458,705 - -0.4 458,738 + TX73_002165 0.13 -0.2 458,754 + TX73_002165 0.15 +0.2 458,769 + TX73_002165 0.17 +0.7 458,769 + TX73_002165 0.17 -0.3 458,777 - TX73_002165 0.18 +1.6 458,777 - TX73_002165 0.18 -0.5 458,777 - TX73_002165 0.18 +0.1 458,782 - TX73_002165 0.18 -1.9 458,784 - TX73_002165 0.19 +0.6 458,799 - TX73_002165 0.20 -0.3 458,837 + TX73_002165 0.25 -1.3 458,845 - TX73_002165 0.26 -1.3 458,846 - TX73_002165 0.26 +1.2 458,855 + TX73_002165 0.27 -0.5 458,865 + TX73_002165 0.29 +1.3 458,865 + TX73_002165 0.29 +1.4 458,868 + TX73_002165 0.29 -1.5 458,885 - TX73_002165 0.31 +1.4 458,887 + TX73_002165 0.31 -0.5 458,887 + TX73_002165 0.31 -0.2 458,887 + TX73_002165 0.31 -0.3 458,889 + TX73_002165 0.31 +0.1 458,895 - TX73_002165 0.32 -0.2 458,895 - TX73_002165 0.32 +1.3 458,895 - TX73_002165 0.32 -0.3 458,934 + TX73_002165 0.37 -0.8 458,940 - TX73_002165 0.38 -0.2 458,940 - TX73_002165 0.38 -0.3 458,946 + TX73_002165 0.38 -0.0 458,976 + TX73_002165 0.42 +1.2 458,984 - TX73_002165 0.43 -0.7 458,990 - TX73_002165 0.44 +0.2 458,995 - TX73_002165 0.45 +0.4 459,021 - TX73_002165 0.48 +1.3 459,024 + TX73_002165 0.48 -0.6 459,032 - TX73_002165 0.49 +1.2 459,032 - TX73_002165 0.49 +1.0 459,032 - TX73_002165 0.49 +0.6 459,034 + TX73_002165 0.49 +0.1 459,042 - TX73_002165 0.50 +0.5 459,042 - TX73_002165 0.50 -1.2 459,042 - TX73_002165 0.50 +0.1 459,060 + TX73_002165 0.53 +0.2 459,068 - TX73_002165 0.54 -1.7 459,068 - TX73_002165 0.54 -0.8 459,068 - TX73_002165 0.54 +0.4 459,085 - TX73_002165 0.56 -1.5 459,094 + TX73_002165 0.57 +0.3 459,094 + TX73_002165 0.57 -0.8 459,102 + TX73_002165 0.58 +0.3 459,110 - TX73_002165 0.59 -1.1 459,145 + TX73_002165 0.63 +0.2 459,217 - TX73_002165 0.72 +1.0 459,217 - TX73_002165 0.72 -1.4 459,217 - TX73_002165 0.72 +0.9 459,222 - TX73_002165 0.72 -0.5 459,238 + TX73_002165 0.74 -1.8 459,276 + TX73_002165 0.79 +1.3 459,367 + -1.1 459,394 - -0.0 459,427 - +0.4 459,443 + +0.1 459,477 - +0.1 459,479 + -1.3 459,484 + +0.8 459,484 + -0.9 459,492 - +1.5 459,543 + -0.3 459,543 + +0.1 459,543 + +1.2 459,543 + -2.4 459,543 + -0.9 459,543 + -1.8 459,551 - +0.1 459,551 - -0.5 459,551 - +0.4 459,551 - -0.6 459,551 - -1.7 459,576 + -1.5 459,576 + +0.4 459,620 - TX73_002170 0.19 +0.7 459,714 - TX73_002170 0.37 -0.3 459,723 - TX73_002170 0.39 +0.2 459,744 + TX73_002170 0.43 +2.9 459,752 - TX73_002170 0.45 +0.0 459,760 + TX73_002170 0.47 +0.1 459,761 + TX73_002170 0.47 -0.3 459,783 + TX73_002170 0.51 +0.2 459,789 + TX73_002170 0.52 +0.8 459,789 + TX73_002170 0.52 +0.7 459,791 - TX73_002170 0.53 -0.3 459,792 - TX73_002170 0.53 +0.5 459,955 + TX73_002170 0.85 +2.1 459,986 + +2.0 460,028 + +0.8 460,051 - -0.8 460,056 - -0.5 460,093 + +0.2 460,095 + -0.6 460,136 - +0.6 460,136 - -0.9 460,230 + TX73_002175 0.12 -0.6 460,230 + TX73_002175 0.12 -0.1 460,238 - TX73_002175 0.13 +0.7 460,306 - TX73_002175 0.22 -0.2 460,345 - TX73_002175 0.27 +0.8 460,349 + TX73_002175 0.27 +0.0 460,364 - TX73_002175 0.29 -2.2 460,370 - TX73_002175 0.30 -0.8 460,504 - TX73_002175 0.47 -0.8 460,529 + TX73_002175 0.50 +0.6 460,628 - TX73_002175 0.62 -0.2 460,662 + TX73_002175 0.67 -1.2 460,663 + TX73_002175 0.67 -0.1 460,699 + TX73_002175 0.71 -0.1 460,706 - TX73_002175 0.72 -0.8 460,707 - TX73_002175 0.72 +0.8 460,709 - TX73_002175 0.72 -0.9 460,775 + TX73_002175 0.81 +0.2 460,796 + TX73_002175 0.83 +1.1 460,804 - TX73_002175 0.84 -0.1 460,929 + -0.4 460,929 + -2.7 460,932 - +0.5 460,932 - +1.2 460,934 + +0.2 460,934 + -1.3 460,939 + -1.7 460,939 + -0.3 460,939 + +0.8 460,939 + +0.4 460,939 + +0.3 460,939 + -0.2 460,939 + +0.9 460,939 + -0.1 460,939 + -2.0 460,939 + +0.3 460,939 + +0.1 460,939 + -0.1 460,939 + +0.9 460,939 + -0.4 460,939 + -0.3 460,939 + +0.1 460,947 - +1.2 460,947 - +1.1 460,947 - +0.1 460,947 - +0.4 460,947 - +1.2 460,947 - +0.3 460,947 - +0.6 460,947 - -1.2 460,947 - +1.2 460,947 - +0.5 460,947 - +0.6 460,962 - -1.1 460,970 + +0.8 461,005 + +1.0
Or see this region's nucleotide sequence