Experiment: NFM with pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_000545 and TX73_000550 are separated by 19 nucleotides TX73_000550 and TX73_000555 are separated by 29 nucleotides
TX73_000545: TX73_000545 - ABC transporter substrate-binding protein, at 121,313 to 122,521
_000545
TX73_000550: TX73_000550 - zinc-binding dehydrogenase, at 122,541 to 123,581
_000550
TX73_000555: TX73_000555 - thiamine pyrophosphate-binding protein, at 123,611 to 125,359
_000555
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 121.602 kb on - strand, within TX73_000545 at 121.624 kb on + strand, within TX73_000545 at 121.714 kb on + strand, within TX73_000545 at 121.722 kb on - strand, within TX73_000545 at 121.802 kb on - strand, within TX73_000545 at 121.804 kb on + strand, within TX73_000545 at 121.820 kb on - strand, within TX73_000545 at 121.855 kb on - strand, within TX73_000545 at 121.857 kb on - strand, within TX73_000545 at 121.873 kb on + strand, within TX73_000545 at 122.040 kb on + strand, within TX73_000545 at 122.094 kb on - strand, within TX73_000545 at 122.255 kb on - strand, within TX73_000545 at 122.299 kb on - strand, within TX73_000545 at 122.307 kb on - strand, within TX73_000545 at 122.352 kb on - strand, within TX73_000545 at 122.371 kb on + strand, within TX73_000545 at 122.441 kb on + strand at 122.494 kb on - strand at 122.615 kb on - strand at 122.631 kb on + strand at 122.788 kb on + strand, within TX73_000550 at 122.851 kb on - strand, within TX73_000550 at 123.103 kb on - strand, within TX73_000550 at 123.139 kb on + strand, within TX73_000550 at 123.154 kb on + strand, within TX73_000550 at 123.250 kb on + strand, within TX73_000550 at 123.268 kb on + strand, within TX73_000550 at 123.365 kb on + strand, within TX73_000550 at 123.416 kb on - strand, within TX73_000550 at 123.453 kb on + strand, within TX73_000550 at 123.458 kb on + strand, within TX73_000550 at 123.466 kb on - strand, within TX73_000550 at 123.574 kb on + strand at 123.574 kb on + strand at 123.594 kb on + strand at 123.597 kb on - strand at 123.599 kb on + strand at 123.599 kb on + strand at 123.599 kb on + strand at 123.599 kb on + strand at 123.607 kb on - strand at 123.607 kb on - strand at 123.614 kb on + strand at 123.617 kb on - strand at 123.645 kb on - strand at 123.714 kb on - strand at 123.855 kb on - strand, within TX73_000555 at 123.891 kb on + strand, within TX73_000555 at 123.943 kb on + strand, within TX73_000555 at 124.003 kb on + strand, within TX73_000555 at 124.017 kb on + strand, within TX73_000555 at 124.035 kb on - strand, within TX73_000555 at 124.057 kb on - strand, within TX73_000555 at 124.083 kb on + strand, within TX73_000555 at 124.084 kb on + strand, within TX73_000555 at 124.091 kb on - strand, within TX73_000555 at 124.118 kb on - strand, within TX73_000555 at 124.235 kb on - strand, within TX73_000555 at 124.281 kb on - strand, within TX73_000555 at 124.447 kb on + strand, within TX73_000555 at 124.479 kb on + strand, within TX73_000555 at 124.503 kb on - strand, within TX73_000555
Per-strain Table
Position Strand Gene LocusTag Fraction NFM with pyruvate remove 121,602 - TX73_000545 0.24 +1.0 121,624 + TX73_000545 0.26 -1.1 121,714 + TX73_000545 0.33 +0.1 121,722 - TX73_000545 0.34 +0.8 121,802 - TX73_000545 0.40 +0.7 121,804 + TX73_000545 0.41 +0.6 121,820 - TX73_000545 0.42 -2.9 121,855 - TX73_000545 0.45 -0.7 121,857 - TX73_000545 0.45 -1.0 121,873 + TX73_000545 0.46 +0.7 122,040 + TX73_000545 0.60 -0.4 122,094 - TX73_000545 0.65 -0.6 122,255 - TX73_000545 0.78 +1.1 122,299 - TX73_000545 0.82 -0.8 122,307 - TX73_000545 0.82 -0.7 122,352 - TX73_000545 0.86 -0.2 122,371 + TX73_000545 0.88 -1.0 122,441 + +1.7 122,494 - -0.2 122,615 - +1.0 122,631 + +1.5 122,788 + TX73_000550 0.24 +0.0 122,851 - TX73_000550 0.30 +0.1 123,103 - TX73_000550 0.54 +0.1 123,139 + TX73_000550 0.57 +0.4 123,154 + TX73_000550 0.59 +0.2 123,250 + TX73_000550 0.68 +0.1 123,268 + TX73_000550 0.70 -0.4 123,365 + TX73_000550 0.79 +1.0 123,416 - TX73_000550 0.84 -1.2 123,453 + TX73_000550 0.88 -0.8 123,458 + TX73_000550 0.88 -0.0 123,466 - TX73_000550 0.89 +0.7 123,574 + -0.4 123,574 + -0.9 123,594 + +0.4 123,597 - -0.8 123,599 + -0.0 123,599 + +0.1 123,599 + -0.1 123,599 + +0.1 123,607 - +0.3 123,607 - +0.6 123,614 + -0.3 123,617 - -0.7 123,645 - +0.6 123,714 - +1.1 123,855 - TX73_000555 0.14 +0.3 123,891 + TX73_000555 0.16 +0.6 123,943 + TX73_000555 0.19 +2.0 124,003 + TX73_000555 0.22 +0.1 124,017 + TX73_000555 0.23 +0.3 124,035 - TX73_000555 0.24 -0.3 124,057 - TX73_000555 0.26 +1.0 124,083 + TX73_000555 0.27 +0.8 124,084 + TX73_000555 0.27 -1.1 124,091 - TX73_000555 0.27 -0.4 124,118 - TX73_000555 0.29 +0.4 124,235 - TX73_000555 0.36 +0.8 124,281 - TX73_000555 0.38 +0.3 124,447 + TX73_000555 0.48 +0.7 124,479 + TX73_000555 0.50 +0.6 124,503 - TX73_000555 0.51 +1.3
Or see this region's nucleotide sequence