Experiment: NFM with pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_003550 and TX73_003555 are separated by 49 nucleotides TX73_003555 and TX73_003560 overlap by 20 nucleotides TX73_003560 and TX73_003565 overlap by 4 nucleotides
TX73_003550: TX73_003550 - tripartite tricarboxylate transporter substrate binding protein, at 762,098 to 763,072
_003550
TX73_003555: TX73_003555 - pyridoxamine 5'-phosphate oxidase family protein, at 763,122 to 763,769
_003555
TX73_003560: TX73_003560 - phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN, at 763,750 to 764,334
_003560
TX73_003565: TX73_003565 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 764,331 to 765,485
_003565
Position (kb) 763
764
765 Strain fitness (log2 ratio) -2
-1
0
1
2 at 762.776 kb on + strand, within TX73_003550 at 762.776 kb on + strand, within TX73_003550 at 762.784 kb on - strand, within TX73_003550 at 762.803 kb on - strand, within TX73_003550 at 762.974 kb on - strand, within TX73_003550 at 762.989 kb on - strand at 763.038 kb on + strand at 763.064 kb on - strand at 763.084 kb on + strand at 763.092 kb on - strand at 763.144 kb on + strand at 763.161 kb on + strand at 763.169 kb on - strand at 763.185 kb on + strand at 763.193 kb on - strand, within TX73_003555 at 763.267 kb on + strand, within TX73_003555 at 763.270 kb on - strand, within TX73_003555 at 763.383 kb on + strand, within TX73_003555 at 763.507 kb on + strand, within TX73_003555 at 763.524 kb on + strand, within TX73_003555 at 763.572 kb on + strand, within TX73_003555 at 763.573 kb on - strand, within TX73_003555 at 763.580 kb on + strand, within TX73_003555 at 763.580 kb on + strand, within TX73_003555 at 763.580 kb on - strand, within TX73_003555 at 763.628 kb on + strand, within TX73_003555 at 763.628 kb on + strand, within TX73_003555 at 763.636 kb on - strand, within TX73_003555 at 763.638 kb on + strand, within TX73_003555 at 763.698 kb on - strand, within TX73_003555 at 763.756 kb on - strand at 763.789 kb on + strand at 763.798 kb on - strand at 763.853 kb on - strand, within TX73_003560 at 763.865 kb on - strand, within TX73_003560 at 763.947 kb on - strand, within TX73_003560 at 763.983 kb on + strand, within TX73_003560 at 763.995 kb on + strand, within TX73_003560 at 764.022 kb on - strand, within TX73_003560 at 764.063 kb on - strand, within TX73_003560 at 764.078 kb on + strand, within TX73_003560 at 764.078 kb on + strand, within TX73_003560 at 764.078 kb on + strand, within TX73_003560 at 764.086 kb on - strand, within TX73_003560 at 764.096 kb on - strand, within TX73_003560 at 764.123 kb on - strand, within TX73_003560 at 764.161 kb on + strand, within TX73_003560 at 764.236 kb on - strand, within TX73_003560 at 764.277 kb on - strand at 764.338 kb on - strand at 764.408 kb on - strand at 764.461 kb on + strand, within TX73_003565 at 764.603 kb on - strand, within TX73_003565 at 764.631 kb on - strand, within TX73_003565 at 764.651 kb on - strand, within TX73_003565 at 764.652 kb on - strand, within TX73_003565 at 764.685 kb on - strand, within TX73_003565 at 764.736 kb on + strand, within TX73_003565 at 764.765 kb on - strand, within TX73_003565 at 764.842 kb on + strand, within TX73_003565 at 764.944 kb on - strand, within TX73_003565 at 765.026 kb on - strand, within TX73_003565 at 765.033 kb on - strand, within TX73_003565 at 765.194 kb on - strand, within TX73_003565 at 765.270 kb on - strand, within TX73_003565 at 765.286 kb on + strand, within TX73_003565 at 765.307 kb on + strand, within TX73_003565 at 765.325 kb on - strand, within TX73_003565
Per-strain Table
Position Strand Gene LocusTag Fraction NFM with pyruvate remove 762,776 + TX73_003550 0.70 -0.4 762,776 + TX73_003550 0.70 +1.5 762,784 - TX73_003550 0.70 -0.1 762,803 - TX73_003550 0.72 -1.6 762,974 - TX73_003550 0.90 -0.4 762,989 - +0.7 763,038 + -0.3 763,064 - +0.4 763,084 + +0.1 763,092 - -0.5 763,144 + -0.2 763,161 + -1.9 763,169 - +0.7 763,185 + -0.1 763,193 - TX73_003555 0.11 -0.7 763,267 + TX73_003555 0.22 -0.4 763,270 - TX73_003555 0.23 +0.5 763,383 + TX73_003555 0.40 -1.0 763,507 + TX73_003555 0.59 -1.1 763,524 + TX73_003555 0.62 -0.2 763,572 + TX73_003555 0.69 +1.1 763,573 - TX73_003555 0.70 -0.7 763,580 + TX73_003555 0.71 +0.5 763,580 + TX73_003555 0.71 -0.4 763,580 - TX73_003555 0.71 -0.4 763,628 + TX73_003555 0.78 -0.1 763,628 + TX73_003555 0.78 -0.6 763,636 - TX73_003555 0.79 -0.5 763,638 + TX73_003555 0.80 -0.6 763,698 - TX73_003555 0.89 -0.5 763,756 - +0.9 763,789 + -0.8 763,798 - +0.3 763,853 - TX73_003560 0.18 +0.3 763,865 - TX73_003560 0.20 -0.8 763,947 - TX73_003560 0.34 +1.6 763,983 + TX73_003560 0.40 -0.6 763,995 + TX73_003560 0.42 +1.6 764,022 - TX73_003560 0.46 +0.1 764,063 - TX73_003560 0.54 +0.7 764,078 + TX73_003560 0.56 -0.8 764,078 + TX73_003560 0.56 +0.0 764,078 + TX73_003560 0.56 -0.7 764,086 - TX73_003560 0.57 +1.2 764,096 - TX73_003560 0.59 -0.3 764,123 - TX73_003560 0.64 +1.4 764,161 + TX73_003560 0.70 -1.4 764,236 - TX73_003560 0.83 +1.6 764,277 - -1.4 764,338 - -0.9 764,408 - -0.1 764,461 + TX73_003565 0.11 -0.8 764,603 - TX73_003565 0.24 -0.9 764,631 - TX73_003565 0.26 +0.9 764,651 - TX73_003565 0.28 -0.1 764,652 - TX73_003565 0.28 +1.3 764,685 - TX73_003565 0.31 -0.0 764,736 + TX73_003565 0.35 +1.7 764,765 - TX73_003565 0.38 -0.1 764,842 + TX73_003565 0.44 +0.3 764,944 - TX73_003565 0.53 -0.0 765,026 - TX73_003565 0.60 +0.2 765,033 - TX73_003565 0.61 -1.7 765,194 - TX73_003565 0.75 +1.9 765,270 - TX73_003565 0.81 -2.1 765,286 + TX73_003565 0.83 -1.7 765,307 + TX73_003565 0.85 -1.4 765,325 - TX73_003565 0.86 +0.6
Or see this region's nucleotide sequence