Experiment: NFM with succinate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_003240 and TX73_003245 are separated by 184 nucleotides TX73_003245 and TX73_003250 overlap by 4 nucleotides TX73_003250 and TX73_003255 are separated by 122 nucleotides
TX73_003240: TX73_003240 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 693,298 to 694,143
_003240
TX73_003245: TX73_003245 - pyrimidine 5'-nucleotidase, at 694,328 to 695,029
_003245
TX73_003250: TX73_003250 - DUF1036 domain-containing protein, at 695,026 to 696,024
_003250
TX73_003255: TX73_003255 - acetylglutamate kinase, at 696,147 to 697,043
_003255
Position (kb) 695
696
697 Strain fitness (log2 ratio) -2
-1
0
1
2 at 694.129 kb on - strand at 694.178 kb on + strand at 694.186 kb on - strand at 694.188 kb on + strand at 694.198 kb on + strand at 694.213 kb on + strand at 694.221 kb on - strand at 694.232 kb on + strand at 694.248 kb on - strand at 694.275 kb on - strand at 694.307 kb on + strand at 694.350 kb on + strand at 694.358 kb on + strand at 694.359 kb on + strand at 694.437 kb on - strand, within TX73_003245 at 694.466 kb on + strand, within TX73_003245 at 694.467 kb on - strand, within TX73_003245 at 694.509 kb on + strand, within TX73_003245 at 694.529 kb on + strand, within TX73_003245 at 694.534 kb on + strand, within TX73_003245 at 694.566 kb on - strand, within TX73_003245 at 694.657 kb on - strand, within TX73_003245 at 694.690 kb on + strand, within TX73_003245 at 694.723 kb on - strand, within TX73_003245 at 694.961 kb on - strand at 694.999 kb on + strand at 695.007 kb on - strand at 695.049 kb on - strand at 695.072 kb on - strand at 695.090 kb on - strand at 695.103 kb on - strand at 695.103 kb on - strand at 695.144 kb on + strand, within TX73_003250 at 695.144 kb on + strand, within TX73_003250 at 695.185 kb on + strand, within TX73_003250 at 695.187 kb on + strand, within TX73_003250 at 695.187 kb on + strand, within TX73_003250 at 695.220 kb on - strand, within TX73_003250 at 695.231 kb on - strand, within TX73_003250 at 695.251 kb on - strand, within TX73_003250 at 695.263 kb on - strand, within TX73_003250 at 695.282 kb on - strand, within TX73_003250 at 695.312 kb on + strand, within TX73_003250 at 695.325 kb on - strand, within TX73_003250 at 695.337 kb on - strand, within TX73_003250 at 695.377 kb on + strand, within TX73_003250 at 695.408 kb on + strand, within TX73_003250 at 695.433 kb on - strand, within TX73_003250 at 695.467 kb on + strand, within TX73_003250 at 695.550 kb on + strand, within TX73_003250 at 695.573 kb on + strand, within TX73_003250 at 695.573 kb on + strand, within TX73_003250 at 695.648 kb on + strand, within TX73_003250 at 695.648 kb on + strand, within TX73_003250 at 695.714 kb on + strand, within TX73_003250 at 695.744 kb on + strand, within TX73_003250 at 695.744 kb on + strand, within TX73_003250 at 695.744 kb on + strand, within TX73_003250 at 695.747 kb on - strand, within TX73_003250 at 695.752 kb on - strand, within TX73_003250 at 695.763 kb on + strand, within TX73_003250 at 695.858 kb on + strand, within TX73_003250 at 695.858 kb on + strand, within TX73_003250 at 695.890 kb on - strand, within TX73_003250 at 695.933 kb on + strand at 695.933 kb on + strand at 695.933 kb on + strand at 695.933 kb on + strand at 695.943 kb on + strand at 695.956 kb on - strand at 695.971 kb on + strand at 695.977 kb on - strand at 695.991 kb on - strand at 696.030 kb on + strand at 696.030 kb on + strand at 696.038 kb on - strand at 696.038 kb on - strand at 696.098 kb on - strand at 696.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NFM with succinate remove 694,129 - +0.9 694,178 + -0.7 694,186 - +0.5 694,188 + -0.5 694,198 + +1.3 694,213 + -0.1 694,221 - +0.1 694,232 + -0.1 694,248 - +0.2 694,275 - +0.2 694,307 + +1.2 694,350 + -0.2 694,358 + -0.1 694,359 + +0.3 694,437 - TX73_003245 0.16 +0.5 694,466 + TX73_003245 0.20 +1.5 694,467 - TX73_003245 0.20 +0.9 694,509 + TX73_003245 0.26 -0.2 694,529 + TX73_003245 0.29 -0.6 694,534 + TX73_003245 0.29 +0.2 694,566 - TX73_003245 0.34 +0.0 694,657 - TX73_003245 0.47 -0.1 694,690 + TX73_003245 0.52 +0.7 694,723 - TX73_003245 0.56 +0.2 694,961 - -0.4 694,999 + -0.5 695,007 - -0.4 695,049 - -0.1 695,072 - +0.5 695,090 - -0.4 695,103 - +0.5 695,103 - +0.0 695,144 + TX73_003250 0.12 -1.0 695,144 + TX73_003250 0.12 -0.3 695,185 + TX73_003250 0.16 -0.2 695,187 + TX73_003250 0.16 -0.6 695,187 + TX73_003250 0.16 +0.1 695,220 - TX73_003250 0.19 +0.7 695,231 - TX73_003250 0.21 +0.3 695,251 - TX73_003250 0.23 +0.3 695,263 - TX73_003250 0.24 +1.5 695,282 - TX73_003250 0.26 +0.5 695,312 + TX73_003250 0.29 +2.2 695,325 - TX73_003250 0.30 -0.4 695,337 - TX73_003250 0.31 +0.4 695,377 + TX73_003250 0.35 -0.0 695,408 + TX73_003250 0.38 +0.7 695,433 - TX73_003250 0.41 -0.2 695,467 + TX73_003250 0.44 +0.1 695,550 + TX73_003250 0.52 +0.5 695,573 + TX73_003250 0.55 -0.4 695,573 + TX73_003250 0.55 +0.1 695,648 + TX73_003250 0.62 +0.1 695,648 + TX73_003250 0.62 -0.2 695,714 + TX73_003250 0.69 +0.1 695,744 + TX73_003250 0.72 +0.6 695,744 + TX73_003250 0.72 -0.2 695,744 + TX73_003250 0.72 +1.7 695,747 - TX73_003250 0.72 -0.2 695,752 - TX73_003250 0.73 +0.1 695,763 + TX73_003250 0.74 +0.9 695,858 + TX73_003250 0.83 -0.8 695,858 + TX73_003250 0.83 +0.0 695,890 - TX73_003250 0.86 -0.6 695,933 + -0.0 695,933 + -0.1 695,933 + -0.2 695,933 + +0.3 695,943 + -0.5 695,956 - -1.9 695,971 + +0.6 695,977 - +0.6 695,991 - -0.9 696,030 + -1.0 696,030 + +0.3 696,038 - +0.4 696,038 - +0.2 696,098 - +0.1 696,108 - +0.2
Or see this region's nucleotide sequence