Experiment: NFM with succinate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_000640 and TX73_000645 overlap by 4 nucleotides TX73_000645 and TX73_000650 are separated by 64 nucleotides TX73_000650 and TX73_000655 are separated by 22 nucleotides TX73_000655 and TX73_000660 overlap by 4 nucleotides
TX73_000640: TX73_000640 - phosphodiesterase, at 140,653 to 141,477
_000640
TX73_000645: TX73_000645 - phosphodiesterase, at 141,474 to 142,298
_000645
TX73_000650: TX73_000650 - ABC transporter substrate-binding protein, at 142,363 to 143,643
_000650
TX73_000655: TX73_000655 - carbohydrate ABC transporter permease, at 143,666 to 144,502
_000655
TX73_000660: TX73_000660 - sugar ABC transporter permease, at 144,499 to 145,443
_000660
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-1
0
1 at 141.371 kb on + strand, within TX73_000640 at 141.379 kb on - strand, within TX73_000640 at 141.379 kb on - strand, within TX73_000640 at 141.392 kb on + strand, within TX73_000640 at 141.395 kb on - strand at 141.407 kb on + strand at 141.503 kb on - strand at 141.598 kb on + strand, within TX73_000645 at 141.646 kb on - strand, within TX73_000645 at 141.695 kb on - strand, within TX73_000645 at 141.696 kb on + strand, within TX73_000645 at 141.749 kb on - strand, within TX73_000645 at 141.907 kb on - strand, within TX73_000645 at 141.912 kb on - strand, within TX73_000645 at 141.925 kb on - strand, within TX73_000645 at 141.994 kb on + strand, within TX73_000645 at 141.994 kb on + strand, within TX73_000645 at 141.994 kb on + strand, within TX73_000645 at 141.994 kb on + strand, within TX73_000645 at 141.994 kb on + strand, within TX73_000645 at 142.004 kb on + strand, within TX73_000645 at 142.103 kb on + strand, within TX73_000645 at 142.180 kb on - strand, within TX73_000645 at 142.196 kb on + strand, within TX73_000645 at 142.322 kb on + strand at 142.382 kb on + strand at 142.395 kb on + strand at 142.415 kb on + strand at 142.460 kb on - strand at 142.498 kb on + strand, within TX73_000650 at 142.616 kb on + strand, within TX73_000650 at 142.624 kb on - strand, within TX73_000650 at 142.674 kb on - strand, within TX73_000650 at 142.676 kb on + strand, within TX73_000650 at 142.684 kb on - strand, within TX73_000650 at 142.684 kb on - strand, within TX73_000650 at 142.723 kb on - strand, within TX73_000650 at 142.792 kb on + strand, within TX73_000650 at 142.939 kb on + strand, within TX73_000650 at 142.971 kb on + strand, within TX73_000650 at 143.143 kb on + strand, within TX73_000650 at 143.174 kb on + strand, within TX73_000650 at 143.337 kb on + strand, within TX73_000650 at 143.374 kb on + strand, within TX73_000650 at 143.382 kb on - strand, within TX73_000650 at 143.382 kb on - strand, within TX73_000650 at 143.411 kb on - strand, within TX73_000650 at 143.425 kb on - strand, within TX73_000650 at 143.430 kb on + strand, within TX73_000650 at 143.430 kb on - strand, within TX73_000650 at 143.438 kb on - strand, within TX73_000650 at 143.471 kb on - strand, within TX73_000650 at 143.541 kb on + strand at 143.573 kb on - strand at 143.598 kb on - strand at 143.660 kb on - strand at 143.683 kb on + strand at 143.826 kb on + strand, within TX73_000655 at 143.826 kb on + strand, within TX73_000655 at 144.017 kb on - strand, within TX73_000655 at 144.044 kb on - strand, within TX73_000655 at 144.189 kb on + strand, within TX73_000655 at 144.243 kb on - strand, within TX73_000655 at 144.332 kb on - strand, within TX73_000655 at 144.369 kb on + strand, within TX73_000655 at 144.377 kb on - strand, within TX73_000655 at 144.396 kb on - strand, within TX73_000655 at 144.408 kb on + strand, within TX73_000655 at 144.428 kb on + strand at 144.446 kb on + strand at 144.484 kb on - strand at 144.565 kb on + strand at 144.576 kb on - strand at 144.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NFM with succinate remove 141,371 + TX73_000640 0.87 -0.2 141,379 - TX73_000640 0.88 -0.2 141,379 - TX73_000640 0.88 +0.6 141,392 + TX73_000640 0.90 -0.5 141,395 - -0.2 141,407 + +0.7 141,503 - +0.3 141,598 + TX73_000645 0.15 +0.1 141,646 - TX73_000645 0.21 +0.2 141,695 - TX73_000645 0.27 -0.4 141,696 + TX73_000645 0.27 +0.8 141,749 - TX73_000645 0.33 +0.4 141,907 - TX73_000645 0.52 -0.1 141,912 - TX73_000645 0.53 +0.3 141,925 - TX73_000645 0.55 -0.6 141,994 + TX73_000645 0.63 -1.5 141,994 + TX73_000645 0.63 +0.5 141,994 + TX73_000645 0.63 -0.1 141,994 + TX73_000645 0.63 +0.5 141,994 + TX73_000645 0.63 +0.4 142,004 + TX73_000645 0.64 -0.1 142,103 + TX73_000645 0.76 -0.1 142,180 - TX73_000645 0.86 +0.4 142,196 + TX73_000645 0.88 +0.2 142,322 + +0.8 142,382 + +0.5 142,395 + -0.1 142,415 + +0.6 142,460 - -0.7 142,498 + TX73_000650 0.11 +0.6 142,616 + TX73_000650 0.20 +0.8 142,624 - TX73_000650 0.20 +0.2 142,674 - TX73_000650 0.24 -0.0 142,676 + TX73_000650 0.24 -0.2 142,684 - TX73_000650 0.25 +0.5 142,684 - TX73_000650 0.25 +0.5 142,723 - TX73_000650 0.28 +0.1 142,792 + TX73_000650 0.33 -1.0 142,939 + TX73_000650 0.45 -0.4 142,971 + TX73_000650 0.47 -0.1 143,143 + TX73_000650 0.61 -1.1 143,174 + TX73_000650 0.63 +0.9 143,337 + TX73_000650 0.76 -0.7 143,374 + TX73_000650 0.79 +0.0 143,382 - TX73_000650 0.80 -0.3 143,382 - TX73_000650 0.80 +0.6 143,411 - TX73_000650 0.82 +0.8 143,425 - TX73_000650 0.83 +0.1 143,430 + TX73_000650 0.83 +0.1 143,430 - TX73_000650 0.83 +0.1 143,438 - TX73_000650 0.84 -0.8 143,471 - TX73_000650 0.86 +0.3 143,541 + +1.4 143,573 - -0.5 143,598 - -0.0 143,660 - -1.1 143,683 + -0.8 143,826 + TX73_000655 0.19 +0.3 143,826 + TX73_000655 0.19 -1.0 144,017 - TX73_000655 0.42 -1.4 144,044 - TX73_000655 0.45 +1.3 144,189 + TX73_000655 0.62 +0.4 144,243 - TX73_000655 0.69 +0.8 144,332 - TX73_000655 0.80 +0.2 144,369 + TX73_000655 0.84 +0.4 144,377 - TX73_000655 0.85 +0.4 144,396 - TX73_000655 0.87 +0.1 144,408 + TX73_000655 0.89 +0.5 144,428 + +1.1 144,446 + +0.2 144,484 - -0.8 144,565 + -1.0 144,576 - -0.2 144,590 - -0.7
Or see this region's nucleotide sequence