Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14465

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14455 and ECOLIN_RS14460 overlap by 11 nucleotidesECOLIN_RS14460 and ECOLIN_RS14465 are separated by 164 nucleotidesECOLIN_RS14465 and ECOLIN_RS14470 are separated by 575 nucleotides ECOLIN_RS14455: ECOLIN_RS14455 - two-component system response regulator GlrR, at 2,922,383 to 2,923,717 _RS14455 ECOLIN_RS14460: ECOLIN_RS14460 - two-component system QseEF-associated lipoprotein QseG, at 2,923,707 to 2,924,420 _RS14460 ECOLIN_RS14465: ECOLIN_RS14465 - two component system sensor histidine kinase QseE/GlrK, at 2,924,585 to 2,926,012 _RS14465 ECOLIN_RS14470: ECOLIN_RS14470 - phosphoribosylformylglycinamidine synthase, at 2,926,588 to 2,930,475 _RS14470 Position (kb) 2924 2925 2926 2927Strain fitness (log2 ratio) -2 -1 0 1at 2923.870 kb on - strand, within ECOLIN_RS14460at 2924.028 kb on + strand, within ECOLIN_RS14460at 2924.105 kb on + strand, within ECOLIN_RS14460at 2924.433 kb on + strandat 2924.454 kb on + strandat 2924.503 kb on + strandat 2924.656 kb on - strandat 2924.656 kb on - strandat 2924.681 kb on - strandat 2924.838 kb on - strand, within ECOLIN_RS14465at 2925.082 kb on - strand, within ECOLIN_RS14465at 2925.128 kb on - strand, within ECOLIN_RS14465at 2925.493 kb on - strand, within ECOLIN_RS14465at 2925.672 kb on - strand, within ECOLIN_RS14465at 2925.754 kb on - strand, within ECOLIN_RS14465at 2925.893 kb on - strandat 2926.100 kb on - strandat 2926.119 kb on - strandat 2926.211 kb on - strandat 2926.234 kb on + strandat 2926.235 kb on - strandat 2926.235 kb on - strandat 2926.235 kb on - strandat 2926.477 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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2,923,870 - ECOLIN_RS14460 0.23 -1.9
2,924,028 + ECOLIN_RS14460 0.45 -1.3
2,924,105 + ECOLIN_RS14460 0.56 -1.6
2,924,433 + -1.9
2,924,454 + -1.1
2,924,503 + -1.3
2,924,656 - -0.8
2,924,656 - +0.1
2,924,681 - -1.1
2,924,838 - ECOLIN_RS14465 0.18 -1.4
2,925,082 - ECOLIN_RS14465 0.35 -1.6
2,925,128 - ECOLIN_RS14465 0.38 -2.5
2,925,493 - ECOLIN_RS14465 0.64 -0.9
2,925,672 - ECOLIN_RS14465 0.76 -2.5
2,925,754 - ECOLIN_RS14465 0.82 -0.7
2,925,893 - -0.9
2,926,100 - -0.8
2,926,119 - -2.1
2,926,211 - -2.2
2,926,234 + -0.5
2,926,235 - -2.1
2,926,235 - -1.2
2,926,235 - -1.7
2,926,477 + -0.4

Or see this region's nucleotide sequence