Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13450

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13445 and ECOLIN_RS13450 are separated by 98 nucleotidesECOLIN_RS13450 and ECOLIN_RS13455 overlap by 4 nucleotides ECOLIN_RS13445: ECOLIN_RS13445 - 4-phosphoerythronate dehydrogenase PdxB, at 2,690,576 to 2,691,712 _RS13445 ECOLIN_RS13450: ECOLIN_RS13450 - flagella biosynthesis regulator Flk, at 2,691,811 to 2,692,806 _RS13450 ECOLIN_RS13455: ECOLIN_RS13455 - MFS transporter, at 2,692,803 to 2,693,981 _RS13455 Position (kb) 2691 2692 2693Strain fitness (log2 ratio) -1 0 1at 2690.851 kb on + strand, within ECOLIN_RS13445at 2690.926 kb on + strand, within ECOLIN_RS13445at 2691.778 kb on + strandat 2691.778 kb on + strandat 2691.797 kb on + strandat 2692.538 kb on + strand, within ECOLIN_RS13450at 2692.571 kb on - strand, within ECOLIN_RS13450at 2692.571 kb on - strand, within ECOLIN_RS13450at 2693.028 kb on - strand, within ECOLIN_RS13455

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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2,690,851 + ECOLIN_RS13445 0.24 +0.4
2,690,926 + ECOLIN_RS13445 0.31 +0.4
2,691,778 + -0.8
2,691,778 + +0.8
2,691,797 + -0.5
2,692,538 + ECOLIN_RS13450 0.73 +0.2
2,692,571 - ECOLIN_RS13450 0.76 +0.6
2,692,571 - ECOLIN_RS13450 0.76 +0.5
2,693,028 - ECOLIN_RS13455 0.19 -1.0

Or see this region's nucleotide sequence