Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20150

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20145 and ECOLIN_RS20150 are separated by 392 nucleotidesECOLIN_RS20150 and ECOLIN_RS20155 are separated by 178 nucleotides ECOLIN_RS20145: ECOLIN_RS20145 - inner membrane protein YhjD, at 4,045,440 to 4,046,453 _RS20145 ECOLIN_RS20150: ECOLIN_RS20150 - MFS transporter, at 4,046,846 to 4,048,168 _RS20150 ECOLIN_RS20155: ECOLIN_RS20155 - AsmA family protein, at 4,048,347 to 4,050,407 _RS20155 Position (kb) 4046 4047 4048 4049Strain fitness (log2 ratio) -3 -2 -1 0 1at 4046.412 kb on + strandat 4046.509 kb on - strandat 4046.587 kb on + strandat 4046.587 kb on + strandat 4046.587 kb on + strandat 4046.588 kb on - strandat 4046.810 kb on + strandat 4046.846 kb on - strandat 4046.846 kb on - strandat 4046.909 kb on - strandat 4046.909 kb on - strandat 4047.261 kb on + strand, within ECOLIN_RS20150at 4047.261 kb on + strand, within ECOLIN_RS20150at 4047.452 kb on - strand, within ECOLIN_RS20150at 4047.485 kb on + strand, within ECOLIN_RS20150at 4047.486 kb on - strand, within ECOLIN_RS20150at 4047.598 kb on - strand, within ECOLIN_RS20150at 4047.605 kb on - strand, within ECOLIN_RS20150at 4047.614 kb on - strand, within ECOLIN_RS20150at 4047.627 kb on - strand, within ECOLIN_RS20150at 4047.628 kb on + strand, within ECOLIN_RS20150at 4047.629 kb on - strand, within ECOLIN_RS20150at 4047.638 kb on + strand, within ECOLIN_RS20150at 4047.658 kb on + strand, within ECOLIN_RS20150at 4047.659 kb on - strand, within ECOLIN_RS20150at 4047.671 kb on - strand, within ECOLIN_RS20150at 4047.912 kb on + strand, within ECOLIN_RS20150at 4048.005 kb on - strand, within ECOLIN_RS20150at 4048.005 kb on - strand, within ECOLIN_RS20150at 4048.012 kb on - strand, within ECOLIN_RS20150at 4048.043 kb on + strandat 4048.043 kb on + strandat 4048.043 kb on + strandat 4048.044 kb on - strandat 4048.044 kb on - strandat 4048.045 kb on + strandat 4048.046 kb on - strandat 4048.067 kb on - strandat 4048.094 kb on + strandat 4048.094 kb on + strandat 4048.094 kb on + strandat 4048.094 kb on + strandat 4048.095 kb on - strandat 4048.166 kb on + strandat 4048.222 kb on + strandat 4048.342 kb on + strandat 4048.342 kb on + strandat 4048.531 kb on + strandat 4048.532 kb on - strandat 4048.532 kb on - strandat 4048.756 kb on + strand, within ECOLIN_RS20155at 4048.756 kb on + strand, within ECOLIN_RS20155at 4048.757 kb on - strand, within ECOLIN_RS20155at 4048.757 kb on - strand, within ECOLIN_RS20155at 4048.757 kb on - strand, within ECOLIN_RS20155at 4048.757 kb on - strand, within ECOLIN_RS20155at 4048.757 kb on - strand, within ECOLIN_RS20155at 4048.974 kb on - strand, within ECOLIN_RS20155at 4049.094 kb on - strand, within ECOLIN_RS20155

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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4,046,412 + -0.1
4,046,509 - +0.2
4,046,587 + -1.1
4,046,587 + -0.3
4,046,587 + -0.4
4,046,588 - -0.5
4,046,810 + +0.1
4,046,846 - +0.7
4,046,846 - +0.3
4,046,909 - +0.1
4,046,909 - -1.4
4,047,261 + ECOLIN_RS20150 0.31 -0.2
4,047,261 + ECOLIN_RS20150 0.31 -0.1
4,047,452 - ECOLIN_RS20150 0.46 +0.1
4,047,485 + ECOLIN_RS20150 0.48 -0.1
4,047,486 - ECOLIN_RS20150 0.48 -0.0
4,047,598 - ECOLIN_RS20150 0.57 -3.3
4,047,605 - ECOLIN_RS20150 0.57 +0.1
4,047,614 - ECOLIN_RS20150 0.58 +0.1
4,047,627 - ECOLIN_RS20150 0.59 +0.0
4,047,628 + ECOLIN_RS20150 0.59 -0.5
4,047,629 - ECOLIN_RS20150 0.59 -0.0
4,047,638 + ECOLIN_RS20150 0.60 +0.3
4,047,658 + ECOLIN_RS20150 0.61 -0.0
4,047,659 - ECOLIN_RS20150 0.61 +0.1
4,047,671 - ECOLIN_RS20150 0.62 -0.0
4,047,912 + ECOLIN_RS20150 0.81 -0.5
4,048,005 - ECOLIN_RS20150 0.88 -0.2
4,048,005 - ECOLIN_RS20150 0.88 +0.2
4,048,012 - ECOLIN_RS20150 0.88 +0.5
4,048,043 + -0.4
4,048,043 + -0.0
4,048,043 + -0.0
4,048,044 - +0.2
4,048,044 - -0.2
4,048,045 + -0.6
4,048,046 - -0.3
4,048,067 - -1.9
4,048,094 + +0.2
4,048,094 + -0.4
4,048,094 + -0.3
4,048,094 + -0.4
4,048,095 - +0.1
4,048,166 + +0.0
4,048,222 + +0.0
4,048,342 + -0.5
4,048,342 + -0.1
4,048,531 + -0.1
4,048,532 - -0.3
4,048,532 - +0.4
4,048,756 + ECOLIN_RS20155 0.20 -1.3
4,048,756 + ECOLIN_RS20155 0.20 +0.1
4,048,757 - ECOLIN_RS20155 0.20 +0.0
4,048,757 - ECOLIN_RS20155 0.20 -0.4
4,048,757 - ECOLIN_RS20155 0.20 +0.2
4,048,757 - ECOLIN_RS20155 0.20 -0.4
4,048,757 - ECOLIN_RS20155 0.20 -0.2
4,048,974 - ECOLIN_RS20155 0.30 +0.1
4,049,094 - ECOLIN_RS20155 0.36 +0.2

Or see this region's nucleotide sequence