Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18055

Experiment: Bas38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18050 and ECOLIN_RS18055 are separated by 348 nucleotidesECOLIN_RS18055 and ECOLIN_RS18060 are separated by 12 nucleotides ECOLIN_RS18050: ECOLIN_RS18050 - N-acetylglucosamine-6-phosphate deacetylase, at 3,639,156 to 3,640,289 _RS18050 ECOLIN_RS18055: ECOLIN_RS18055 - AgaS family sugar isomerase, at 3,640,638 to 3,641,792 _RS18055 ECOLIN_RS18060: ECOLIN_RS18060 - tagatose-bisphosphate aldolase subunit KbaY, at 3,641,805 to 3,642,665 _RS18060 Position (kb) 3640 3641 3642Strain fitness (log2 ratio) -2 -1 0 1at 3639.696 kb on - strand, within ECOLIN_RS18050at 3639.728 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.795 kb on + strand, within ECOLIN_RS18050at 3639.796 kb on - strand, within ECOLIN_RS18050at 3639.998 kb on + strand, within ECOLIN_RS18050at 3639.998 kb on + strand, within ECOLIN_RS18050at 3639.998 kb on + strand, within ECOLIN_RS18050at 3639.998 kb on + strand, within ECOLIN_RS18050at 3639.999 kb on - strand, within ECOLIN_RS18050at 3640.003 kb on + strand, within ECOLIN_RS18050at 3640.003 kb on + strand, within ECOLIN_RS18050at 3640.004 kb on - strand, within ECOLIN_RS18050at 3640.004 kb on - strand, within ECOLIN_RS18050at 3640.276 kb on + strandat 3640.276 kb on + strandat 3640.747 kb on + strandat 3640.835 kb on + strand, within ECOLIN_RS18055at 3640.929 kb on + strand, within ECOLIN_RS18055at 3640.929 kb on + strand, within ECOLIN_RS18055at 3641.026 kb on + strand, within ECOLIN_RS18055at 3641.166 kb on - strand, within ECOLIN_RS18055at 3641.341 kb on + strand, within ECOLIN_RS18055at 3641.409 kb on + strand, within ECOLIN_RS18055at 3641.410 kb on - strand, within ECOLIN_RS18055at 3641.410 kb on - strand, within ECOLIN_RS18055at 3641.610 kb on - strand, within ECOLIN_RS18055at 3641.610 kb on - strand, within ECOLIN_RS18055at 3641.671 kb on - strand, within ECOLIN_RS18055at 3641.714 kb on + strandat 3641.715 kb on - strandat 3641.715 kb on - strandat 3641.768 kb on - strandat 3641.804 kb on + strandat 3641.804 kb on + strandat 3641.992 kb on - strand, within ECOLIN_RS18060at 3642.021 kb on + strand, within ECOLIN_RS18060at 3642.022 kb on - strand, within ECOLIN_RS18060at 3642.023 kb on + strand, within ECOLIN_RS18060at 3642.352 kb on - strand, within ECOLIN_RS18060at 3642.582 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas38
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3,639,696 - ECOLIN_RS18050 0.48 +0.5
3,639,728 + ECOLIN_RS18050 0.50 -0.4
3,639,795 + ECOLIN_RS18050 0.56 +0.7
3,639,795 + ECOLIN_RS18050 0.56 -0.2
3,639,795 + ECOLIN_RS18050 0.56 +0.1
3,639,795 + ECOLIN_RS18050 0.56 +0.9
3,639,795 + ECOLIN_RS18050 0.56 +0.2
3,639,795 + ECOLIN_RS18050 0.56 -0.1
3,639,796 - ECOLIN_RS18050 0.56 +0.2
3,639,998 + ECOLIN_RS18050 0.74 +0.4
3,639,998 + ECOLIN_RS18050 0.74 -0.2
3,639,998 + ECOLIN_RS18050 0.74 +0.0
3,639,998 + ECOLIN_RS18050 0.74 -0.1
3,639,999 - ECOLIN_RS18050 0.74 +0.6
3,640,003 + ECOLIN_RS18050 0.75 +0.3
3,640,003 + ECOLIN_RS18050 0.75 -0.5
3,640,004 - ECOLIN_RS18050 0.75 +0.0
3,640,004 - ECOLIN_RS18050 0.75 -0.5
3,640,276 + -0.2
3,640,276 + -0.2
3,640,747 + -0.2
3,640,835 + ECOLIN_RS18055 0.17 +0.8
3,640,929 + ECOLIN_RS18055 0.25 -1.9
3,640,929 + ECOLIN_RS18055 0.25 -0.7
3,641,026 + ECOLIN_RS18055 0.34 +0.1
3,641,166 - ECOLIN_RS18055 0.46 -0.2
3,641,341 + ECOLIN_RS18055 0.61 -0.6
3,641,409 + ECOLIN_RS18055 0.67 +0.5
3,641,410 - ECOLIN_RS18055 0.67 -0.8
3,641,410 - ECOLIN_RS18055 0.67 +1.2
3,641,610 - ECOLIN_RS18055 0.84 +0.5
3,641,610 - ECOLIN_RS18055 0.84 -0.7
3,641,671 - ECOLIN_RS18055 0.89 +0.0
3,641,714 + +0.7
3,641,715 - -0.1
3,641,715 - -0.6
3,641,768 - -0.2
3,641,804 + -1.5
3,641,804 + -2.1
3,641,992 - ECOLIN_RS18060 0.22 -0.0
3,642,021 + ECOLIN_RS18060 0.25 +1.7
3,642,022 - ECOLIN_RS18060 0.25 -0.1
3,642,023 + ECOLIN_RS18060 0.25 +0.5
3,642,352 - ECOLIN_RS18060 0.64 -0.6
3,642,582 - -0.9

Or see this region's nucleotide sequence