Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS31590

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16175 and ECOLIN_RS31590 are separated by 290 nucleotidesECOLIN_RS31590 and ECOLIN_RS16180 are separated by 73 nucleotides ECOLIN_RS16175: ECOLIN_RS16175 - glycine cleavage system aminomethyltransferase GcvT, at 3,281,156 to 3,282,250 _RS16175 ECOLIN_RS31590: ECOLIN_RS31590 - hypothetical protein, at 3,282,541 to 3,282,624 _RS31590 ECOLIN_RS16180: ECOLIN_RS16180 - FAD-dependent 2-octaprenylphenol hydroxylase, at 3,282,698 to 3,283,900 _RS16180 Position (kb) 3282 3283Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3281.544 kb on + strand, within ECOLIN_RS16175at 3282.098 kb on - strand, within ECOLIN_RS16175at 3282.127 kb on + strand, within ECOLIN_RS16175at 3282.696 kb on - strandat 3282.696 kb on - strandat 3282.700 kb on - strandat 3282.854 kb on - strand, within ECOLIN_RS16180at 3282.995 kb on + strand, within ECOLIN_RS16180at 3283.125 kb on - strand, within ECOLIN_RS16180

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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3,281,544 + ECOLIN_RS16175 0.35 -0.2
3,282,098 - ECOLIN_RS16175 0.86 -3.6
3,282,127 + ECOLIN_RS16175 0.89 +0.3
3,282,696 - -0.5
3,282,696 - -1.8
3,282,700 - +0.7
3,282,854 - ECOLIN_RS16180 0.13 -0.5
3,282,995 + ECOLIN_RS16180 0.25 -4.4
3,283,125 - ECOLIN_RS16180 0.35 -3.4

Or see this region's nucleotide sequence