Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18535

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18525 and ECOLIN_RS18530 are separated by 15 nucleotidesECOLIN_RS18530 and ECOLIN_RS18535 are separated by 219 nucleotidesECOLIN_RS18535 and ECOLIN_RS18540 are separated by 193 nucleotidesECOLIN_RS18540 and ECOLIN_RS18545 are separated by 153 nucleotides ECOLIN_RS18525: ECOLIN_RS18525 - 30S ribosomal protein S9, at 3,731,640 to 3,732,032 _RS18525 ECOLIN_RS18530: ECOLIN_RS18530 - 50S ribosomal protein L13, at 3,732,048 to 3,732,476 _RS18530 ECOLIN_RS18535: ECOLIN_RS18535 - cell division protein ZapE, at 3,732,696 to 3,733,823 _RS18535 ECOLIN_RS18540: ECOLIN_RS18540 - Z-ring associated protein ZapG, at 3,734,017 to 3,734,415 _RS18540 ECOLIN_RS18545: ECOLIN_RS18545 - serine endoprotease DegQ, at 3,734,569 to 3,735,936 _RS18545 Position (kb) 3732 3733 3734Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 3732.724 kb on + strandat 3732.915 kb on + strand, within ECOLIN_RS18535at 3732.915 kb on + strand, within ECOLIN_RS18535at 3732.916 kb on - strand, within ECOLIN_RS18535at 3732.960 kb on - strand, within ECOLIN_RS18535at 3732.961 kb on + strand, within ECOLIN_RS18535at 3732.961 kb on + strand, within ECOLIN_RS18535at 3732.962 kb on - strand, within ECOLIN_RS18535at 3732.962 kb on - strand, within ECOLIN_RS18535at 3732.962 kb on - strand, within ECOLIN_RS18535at 3732.962 kb on - strand, within ECOLIN_RS18535at 3732.995 kb on + strand, within ECOLIN_RS18535at 3732.998 kb on + strand, within ECOLIN_RS18535at 3732.999 kb on - strand, within ECOLIN_RS18535at 3733.062 kb on + strand, within ECOLIN_RS18535at 3733.107 kb on + strand, within ECOLIN_RS18535at 3733.107 kb on + strand, within ECOLIN_RS18535at 3733.107 kb on + strand, within ECOLIN_RS18535at 3733.108 kb on - strand, within ECOLIN_RS18535at 3733.176 kb on - strand, within ECOLIN_RS18535at 3733.320 kb on - strand, within ECOLIN_RS18535at 3733.320 kb on - strand, within ECOLIN_RS18535at 3733.322 kb on + strand, within ECOLIN_RS18535at 3733.499 kb on + strand, within ECOLIN_RS18535at 3733.598 kb on + strand, within ECOLIN_RS18535at 3733.598 kb on + strand, within ECOLIN_RS18535at 3733.598 kb on + strand, within ECOLIN_RS18535at 3733.599 kb on - strand, within ECOLIN_RS18535at 3733.599 kb on - strand, within ECOLIN_RS18535at 3733.599 kb on - strand, within ECOLIN_RS18535at 3733.599 kb on - strand, within ECOLIN_RS18535at 3733.600 kb on + strand, within ECOLIN_RS18535at 3733.600 kb on + strand, within ECOLIN_RS18535at 3733.600 kb on + strand, within ECOLIN_RS18535at 3733.601 kb on - strand, within ECOLIN_RS18535at 3733.601 kb on - strand, within ECOLIN_RS18535at 3733.601 kb on - strand, within ECOLIN_RS18535at 3733.601 kb on - strand, within ECOLIN_RS18535at 3733.601 kb on - strand, within ECOLIN_RS18535at 3733.602 kb on + strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.603 kb on - strand, within ECOLIN_RS18535at 3733.619 kb on + strand, within ECOLIN_RS18535at 3733.619 kb on + strand, within ECOLIN_RS18535at 3733.829 kb on + strandat 3733.896 kb on + strandat 3733.941 kb on - strandat 3733.942 kb on + strandat 3733.943 kb on - strandat 3734.029 kb on + strandat 3734.036 kb on + strandat 3734.094 kb on + strand, within ECOLIN_RS18540at 3734.095 kb on - strand, within ECOLIN_RS18540at 3734.099 kb on + strand, within ECOLIN_RS18540at 3734.099 kb on + strand, within ECOLIN_RS18540at 3734.607 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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3,732,724 + +0.1
3,732,915 + ECOLIN_RS18535 0.19 +0.4
3,732,915 + ECOLIN_RS18535 0.19 +1.7
3,732,916 - ECOLIN_RS18535 0.20 +0.8
3,732,960 - ECOLIN_RS18535 0.23 -0.0
3,732,961 + ECOLIN_RS18535 0.23 +0.1
3,732,961 + ECOLIN_RS18535 0.23 -0.1
3,732,962 - ECOLIN_RS18535 0.24 +0.0
3,732,962 - ECOLIN_RS18535 0.24 -0.3
3,732,962 - ECOLIN_RS18535 0.24 +0.2
3,732,962 - ECOLIN_RS18535 0.24 +0.1
3,732,995 + ECOLIN_RS18535 0.27 -0.2
3,732,998 + ECOLIN_RS18535 0.27 +0.1
3,732,999 - ECOLIN_RS18535 0.27 -0.1
3,733,062 + ECOLIN_RS18535 0.32 -0.3
3,733,107 + ECOLIN_RS18535 0.36 +0.3
3,733,107 + ECOLIN_RS18535 0.36 -0.0
3,733,107 + ECOLIN_RS18535 0.36 +1.1
3,733,108 - ECOLIN_RS18535 0.37 +0.7
3,733,176 - ECOLIN_RS18535 0.43 +0.2
3,733,320 - ECOLIN_RS18535 0.55 +1.2
3,733,320 - ECOLIN_RS18535 0.55 -0.6
3,733,322 + ECOLIN_RS18535 0.55 -0.4
3,733,499 + ECOLIN_RS18535 0.71 +1.1
3,733,598 + ECOLIN_RS18535 0.80 -0.1
3,733,598 + ECOLIN_RS18535 0.80 -1.3
3,733,598 + ECOLIN_RS18535 0.80 +0.2
3,733,599 - ECOLIN_RS18535 0.80 +0.2
3,733,599 - ECOLIN_RS18535 0.80 -0.6
3,733,599 - ECOLIN_RS18535 0.80 +0.1
3,733,599 - ECOLIN_RS18535 0.80 -0.9
3,733,600 + ECOLIN_RS18535 0.80 -0.3
3,733,600 + ECOLIN_RS18535 0.80 +0.2
3,733,600 + ECOLIN_RS18535 0.80 +0.1
3,733,601 - ECOLIN_RS18535 0.80 +0.1
3,733,601 - ECOLIN_RS18535 0.80 +0.1
3,733,601 - ECOLIN_RS18535 0.80 +0.2
3,733,601 - ECOLIN_RS18535 0.80 -0.1
3,733,601 - ECOLIN_RS18535 0.80 +0.3
3,733,602 + ECOLIN_RS18535 0.80 +0.2
3,733,603 - ECOLIN_RS18535 0.80 -2.3
3,733,603 - ECOLIN_RS18535 0.80 -0.4
3,733,603 - ECOLIN_RS18535 0.80 -0.6
3,733,603 - ECOLIN_RS18535 0.80 +0.2
3,733,603 - ECOLIN_RS18535 0.80 -0.3
3,733,603 - ECOLIN_RS18535 0.80 +0.7
3,733,603 - ECOLIN_RS18535 0.80 +0.1
3,733,619 + ECOLIN_RS18535 0.82 +0.1
3,733,619 + ECOLIN_RS18535 0.82 -0.1
3,733,829 + +0.1
3,733,896 + +0.0
3,733,941 - -2.4
3,733,942 + -2.0
3,733,943 - -5.7
3,734,029 + -7.8
3,734,036 + -6.6
3,734,094 + ECOLIN_RS18540 0.19 -6.4
3,734,095 - ECOLIN_RS18540 0.20 -4.2
3,734,099 + ECOLIN_RS18540 0.21 -3.9
3,734,099 + ECOLIN_RS18540 0.21 -3.8
3,734,607 - -0.6

Or see this region's nucleotide sequence