Experiment: Bas37
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS12755 and ECOLIN_RS12760 are separated by 27 nucleotides ECOLIN_RS12760 and ECOLIN_RS12765 are separated by 149 nucleotides ECOLIN_RS12765 and ECOLIN_RS12770 are separated by 124 nucleotides ECOLIN_RS12770 and ECOLIN_RS12775 are separated by 138 nucleotides
ECOLIN_RS12755: ECOLIN_RS12755 - multidrug efflux MFS transporter Bcr, at 2,541,161 to 2,542,351
_RS12755
ECOLIN_RS12760: ECOLIN_RS12760 - 16S rRNA pseudouridine(516) synthase RsuA, at 2,542,379 to 2,543,074
_RS12760
ECOLIN_RS12765: ECOLIN_RS12765 - DEAD/DEAH box helicase, at 2,543,224 to 2,544,984
_RS12765
ECOLIN_RS12770: ECOLIN_RS12770 - 50S ribosomal protein L25, at 2,545,109 to 2,545,393
_RS12770
ECOLIN_RS12775: ECOLIN_RS12775 - nucleoid-associated protein YejK, at 2,545,532 to 2,546,539
_RS12775
Position (kb)
2543
2544
2545 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2542.266 kb on + strand at 2542.267 kb on - strand at 2544.584 kb on + strand, within ECOLIN_RS12765 at 2544.645 kb on - strand, within ECOLIN_RS12765 at 2545.402 kb on + strand at 2545.859 kb on - strand, within ECOLIN_RS12775
Per-strain Table
Position Strand Gene LocusTag Fraction Bas37 remove 2,542,266 + -0.3 2,542,267 - +0.3 2,544,584 + ECOLIN_RS12765 0.77 +2.9 2,544,645 - ECOLIN_RS12765 0.81 +0.5 2,545,402 + -2.6 2,545,859 - ECOLIN_RS12775 0.32 -1.0
Or see this region's nucleotide sequence