Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12765

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12755 and ECOLIN_RS12760 are separated by 27 nucleotidesECOLIN_RS12760 and ECOLIN_RS12765 are separated by 149 nucleotidesECOLIN_RS12765 and ECOLIN_RS12770 are separated by 124 nucleotidesECOLIN_RS12770 and ECOLIN_RS12775 are separated by 138 nucleotides ECOLIN_RS12755: ECOLIN_RS12755 - multidrug efflux MFS transporter Bcr, at 2,541,161 to 2,542,351 _RS12755 ECOLIN_RS12760: ECOLIN_RS12760 - 16S rRNA pseudouridine(516) synthase RsuA, at 2,542,379 to 2,543,074 _RS12760 ECOLIN_RS12765: ECOLIN_RS12765 - DEAD/DEAH box helicase, at 2,543,224 to 2,544,984 _RS12765 ECOLIN_RS12770: ECOLIN_RS12770 - 50S ribosomal protein L25, at 2,545,109 to 2,545,393 _RS12770 ECOLIN_RS12775: ECOLIN_RS12775 - nucleoid-associated protein YejK, at 2,545,532 to 2,546,539 _RS12775 Position (kb) 2543 2544 2545Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2542.266 kb on + strandat 2542.267 kb on - strandat 2544.584 kb on + strand, within ECOLIN_RS12765at 2544.645 kb on - strand, within ECOLIN_RS12765at 2545.402 kb on + strandat 2545.859 kb on - strand, within ECOLIN_RS12775

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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2,542,266 + -0.3
2,542,267 - +0.3
2,544,584 + ECOLIN_RS12765 0.77 +2.9
2,544,645 - ECOLIN_RS12765 0.81 +0.5
2,545,402 + -2.6
2,545,859 - ECOLIN_RS12775 0.32 -1.0

Or see this region's nucleotide sequence