Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02545

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02535 and ECOLIN_RS02540 are separated by 24 nucleotidesECOLIN_RS02540 and ECOLIN_RS02545 overlap by 1 nucleotidesECOLIN_RS02545 and ECOLIN_RS02550 are separated by 205 nucleotides ECOLIN_RS02535: ECOLIN_RS02535 - 1-deoxy-D-xylulose-5-phosphate synthase, at 517,621 to 519,483 _RS02535 ECOLIN_RS02540: ECOLIN_RS02540 - (2E,6E)-farnesyl diphosphate synthase, at 519,508 to 520,407 _RS02540 ECOLIN_RS02545: ECOLIN_RS02545 - exodeoxyribonuclease VII small subunit, at 520,407 to 520,649 _RS02545 ECOLIN_RS02550: ECOLIN_RS02550 - tRNA uracil 4-sulfurtransferase ThiI, at 520,855 to 522,303 _RS02550 Position (kb) 520 521Strain fitness (log2 ratio) -2 -1 0 1at 520.561 kb on - strand, within ECOLIN_RS02545at 520.729 kb on - strandat 520.827 kb on + strandat 520.827 kb on + strandat 520.963 kb on + strandat 521.259 kb on + strand, within ECOLIN_RS02550at 521.259 kb on + strand, within ECOLIN_RS02550at 521.358 kb on + strand, within ECOLIN_RS02550at 521.581 kb on - strand, within ECOLIN_RS02550

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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520,561 - ECOLIN_RS02545 0.63 -2.4
520,729 - -0.2
520,827 + -1.2
520,827 + -0.1
520,963 + +1.7
521,259 + ECOLIN_RS02550 0.28 -0.5
521,259 + ECOLIN_RS02550 0.28 +0.2
521,358 + ECOLIN_RS02550 0.35 +0.1
521,581 - ECOLIN_RS02550 0.50 -1.2

Or see this region's nucleotide sequence