Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00370

Experiment: Bas37

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00365 and ECOLIN_RS00370 overlap by 25 nucleotidesECOLIN_RS00370 and ECOLIN_RS00375 are separated by 163 nucleotides ECOLIN_RS00365: ECOLIN_RS00365 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 75,891 to 77,501 _RS00365 ECOLIN_RS00370: ECOLIN_RS00370 - thiamine ABC transporter substrate binding subunit, at 77,477 to 78,460 _RS00370 ECOLIN_RS00375: ECOLIN_RS00375 - DNA-binding transcriptional regulator SgrR, at 78,624 to 80,279 _RS00375 Position (kb) 77 78 79Strain fitness (log2 ratio) -1 0 1at 76.643 kb on + strand, within ECOLIN_RS00365at 76.644 kb on - strand, within ECOLIN_RS00365at 76.810 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365at 77.028 kb on + strand, within ECOLIN_RS00365at 77.769 kb on + strand, within ECOLIN_RS00370at 77.774 kb on - strand, within ECOLIN_RS00370at 77.818 kb on - strand, within ECOLIN_RS00370at 77.978 kb on - strand, within ECOLIN_RS00370at 78.381 kb on + strandat 78.381 kb on + strandat 78.382 kb on - strandat 78.382 kb on - strandat 78.385 kb on + strandat 78.386 kb on - strandat 78.387 kb on + strandat 78.388 kb on - strandat 78.600 kb on - strandat 78.701 kb on + strandat 78.701 kb on + strandat 78.702 kb on - strandat 78.702 kb on - strandat 78.702 kb on - strandat 78.702 kb on - strandat 78.702 kb on - strandat 78.702 kb on - strandat 79.271 kb on - strand, within ECOLIN_RS00375at 79.274 kb on - strand, within ECOLIN_RS00375

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas37
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76,643 + ECOLIN_RS00365 0.47 +1.0
76,644 - ECOLIN_RS00365 0.47 +0.2
76,810 + ECOLIN_RS00365 0.57 +0.0
76,896 + ECOLIN_RS00365 0.62 -0.3
76,896 + ECOLIN_RS00365 0.62 -0.1
77,028 + ECOLIN_RS00365 0.71 +0.6
77,769 + ECOLIN_RS00370 0.30 +0.0
77,774 - ECOLIN_RS00370 0.30 +0.5
77,818 - ECOLIN_RS00370 0.35 +0.7
77,978 - ECOLIN_RS00370 0.51 +0.4
78,381 + +0.3
78,381 + +0.9
78,382 - +0.7
78,382 - +0.6
78,385 + +0.5
78,386 - -0.5
78,387 + +0.5
78,388 - -0.7
78,600 - +0.0
78,701 + -0.7
78,701 + -0.1
78,702 - +0.3
78,702 - -0.0
78,702 - +0.2
78,702 - +0.4
78,702 - -0.1
78,702 - -0.9
79,271 - ECOLIN_RS00375 0.39 +0.6
79,274 - ECOLIN_RS00375 0.39 -0.2

Or see this region's nucleotide sequence