Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS23565

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS23555 and ECOLIN_RS23560 are separated by 44 nucleotidesECOLIN_RS23560 and ECOLIN_RS23565 overlap by 4 nucleotidesECOLIN_RS23565 and ECOLIN_RS23570 overlap by 4 nucleotidesECOLIN_RS23570 and ECOLIN_RS23575 are separated by 19 nucleotides ECOLIN_RS23555: ECOLIN_RS23555 - ammonia-forming nitrite reductase cytochrome c552 subunit, at 4,748,223 to 4,749,659 _RS23555 ECOLIN_RS23560: ECOLIN_RS23560 - cytochrome c nitrite reductase pentaheme subunit, at 4,749,704 to 4,750,270 _RS23560 ECOLIN_RS23565: ECOLIN_RS23565 - cytochrome c nitrite reductase Fe-S protein, at 4,750,267 to 4,750,938 _RS23565 ECOLIN_RS23570: ECOLIN_RS23570 - cytochrome c nitrite reductase subunit NrfD, at 4,750,935 to 4,751,891 _RS23570 ECOLIN_RS23575: ECOLIN_RS23575 - heme lyase CcmF/NrfE family subunit, at 4,751,911 to 4,753,629 _RS23575 Position (kb) 4750 4751Strain fitness (log2 ratio) -2 -1 0 1 2at 4749.396 kb on - strand, within ECOLIN_RS23555at 4749.703 kb on + strandat 4749.714 kb on + strandat 4749.714 kb on + strandat 4749.715 kb on - strandat 4749.723 kb on + strandat 4749.880 kb on + strand, within ECOLIN_RS23560at 4749.880 kb on + strand, within ECOLIN_RS23560at 4750.014 kb on - strand, within ECOLIN_RS23560at 4750.042 kb on + strand, within ECOLIN_RS23560at 4750.042 kb on + strand, within ECOLIN_RS23560at 4750.043 kb on - strand, within ECOLIN_RS23560at 4750.150 kb on + strand, within ECOLIN_RS23560at 4750.654 kb on + strand, within ECOLIN_RS23565at 4750.914 kb on + strandat 4750.996 kb on - strandat 4751.084 kb on + strand, within ECOLIN_RS23570at 4751.085 kb on - strand, within ECOLIN_RS23570at 4751.085 kb on - strand, within ECOLIN_RS23570at 4751.085 kb on - strand, within ECOLIN_RS23570at 4751.204 kb on + strand, within ECOLIN_RS23570at 4751.205 kb on - strand, within ECOLIN_RS23570at 4751.205 kb on - strand, within ECOLIN_RS23570at 4751.205 kb on - strand, within ECOLIN_RS23570at 4751.244 kb on + strand, within ECOLIN_RS23570at 4751.245 kb on - strand, within ECOLIN_RS23570at 4751.245 kb on - strand, within ECOLIN_RS23570at 4751.418 kb on + strand, within ECOLIN_RS23570at 4751.419 kb on - strand, within ECOLIN_RS23570at 4751.584 kb on + strand, within ECOLIN_RS23570at 4751.585 kb on - strand, within ECOLIN_RS23570at 4751.585 kb on - strand, within ECOLIN_RS23570at 4751.889 kb on + strandat 4751.918 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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4,749,396 - ECOLIN_RS23555 0.82 +0.2
4,749,703 + +0.0
4,749,714 + -0.5
4,749,714 + -1.8
4,749,715 - -0.4
4,749,723 + -0.5
4,749,880 + ECOLIN_RS23560 0.31 -0.4
4,749,880 + ECOLIN_RS23560 0.31 +0.9
4,750,014 - ECOLIN_RS23560 0.55 +0.5
4,750,042 + ECOLIN_RS23560 0.60 +1.8
4,750,042 + ECOLIN_RS23560 0.60 +0.6
4,750,043 - ECOLIN_RS23560 0.60 +0.0
4,750,150 + ECOLIN_RS23560 0.79 +0.1
4,750,654 + ECOLIN_RS23565 0.58 +0.0
4,750,914 + -0.2
4,750,996 - +1.3
4,751,084 + ECOLIN_RS23570 0.16 -0.5
4,751,085 - ECOLIN_RS23570 0.16 -0.4
4,751,085 - ECOLIN_RS23570 0.16 +1.2
4,751,085 - ECOLIN_RS23570 0.16 +0.1
4,751,204 + ECOLIN_RS23570 0.28 +0.0
4,751,205 - ECOLIN_RS23570 0.28 -0.4
4,751,205 - ECOLIN_RS23570 0.28 -0.0
4,751,205 - ECOLIN_RS23570 0.28 -0.5
4,751,244 + ECOLIN_RS23570 0.32 +0.7
4,751,245 - ECOLIN_RS23570 0.32 -0.7
4,751,245 - ECOLIN_RS23570 0.32 +0.2
4,751,418 + ECOLIN_RS23570 0.50 +0.1
4,751,419 - ECOLIN_RS23570 0.51 +0.3
4,751,584 + ECOLIN_RS23570 0.68 +1.1
4,751,585 - ECOLIN_RS23570 0.68 +0.1
4,751,585 - ECOLIN_RS23570 0.68 +0.0
4,751,889 + +0.0
4,751,918 + +0.3

Or see this region's nucleotide sequence