Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS23320

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS23310 and ECOLIN_RS23315 are separated by 178 nucleotidesECOLIN_RS23315 and ECOLIN_RS23320 are separated by 12 nucleotidesECOLIN_RS23320 and ECOLIN_RS23325 are separated by 154 nucleotides ECOLIN_RS23310: ECOLIN_RS23310 - maltose operon protein MalM, at 4,690,612 to 4,691,532 _RS23310 ECOLIN_RS23315: ECOLIN_RS23315 - chorismate lyase, at 4,691,711 to 4,692,208 _RS23315 ECOLIN_RS23320: ECOLIN_RS23320 - 4-hydroxybenzoate octaprenyltransferase, at 4,692,221 to 4,693,093 _RS23320 ECOLIN_RS23325: ECOLIN_RS23325 - glycerol-3-phosphate 1-O-acyltransferase PlsB, at 4,693,248 to 4,695,671 _RS23325 Position (kb) 4692 4693 4694Strain fitness (log2 ratio) -1 0 1at 4691.222 kb on + strand, within ECOLIN_RS23310at 4691.396 kb on - strand, within ECOLIN_RS23310at 4691.400 kb on + strand, within ECOLIN_RS23310at 4691.443 kb on - strandat 4691.530 kb on + strandat 4691.648 kb on - strandat 4691.696 kb on - strandat 4692.105 kb on + strand, within ECOLIN_RS23315at 4692.171 kb on + strandat 4692.204 kb on - strandat 4692.204 kb on - strandat 4692.206 kb on + strandat 4692.207 kb on - strandat 4693.099 kb on + strandat 4693.182 kb on + strandat 4693.299 kb on - strandat 4693.299 kb on - strandat 4693.299 kb on - strandat 4693.299 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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4,691,222 + ECOLIN_RS23310 0.66 +0.1
4,691,396 - ECOLIN_RS23310 0.85 +0.4
4,691,400 + ECOLIN_RS23310 0.86 -0.4
4,691,443 - -0.1
4,691,530 + +0.5
4,691,648 - +0.9
4,691,696 - -1.5
4,692,105 + ECOLIN_RS23315 0.79 +0.1
4,692,171 + -0.1
4,692,204 - -1.4
4,692,204 - -0.4
4,692,206 + +0.1
4,692,207 - -0.3
4,693,099 + +0.2
4,693,182 + +0.2
4,693,299 - -1.3
4,693,299 - -1.2
4,693,299 - -0.1
4,693,299 - +0.2

Or see this region's nucleotide sequence