Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20120

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20115 and ECOLIN_RS20120 are separated by 367 nucleotidesECOLIN_RS20120 and ECOLIN_RS20125 are separated by 210 nucleotides ECOLIN_RS20115: ECOLIN_RS20115 - acid resistance transcriptional activator GadX, at 4,037,065 to 4,037,889 _RS20115 ECOLIN_RS20120: ECOLIN_RS20120 - glutamate decarboxylase, at 4,038,257 to 4,039,657 _RS20120 ECOLIN_RS20125: ECOLIN_RS20125 - cytochrome c peroxidase, at 4,039,868 to 4,041,265 _RS20125 Position (kb) 4038 4039 4040Strain fitness (log2 ratio) -1 0 1at 4037.554 kb on + strand, within ECOLIN_RS20115at 4037.666 kb on - strand, within ECOLIN_RS20115at 4038.043 kb on + strandat 4038.043 kb on + strandat 4038.043 kb on + strandat 4038.147 kb on - strandat 4038.177 kb on + strandat 4038.207 kb on - strandat 4038.269 kb on - strandat 4038.641 kb on - strand, within ECOLIN_RS20120at 4039.739 kb on + strandat 4039.865 kb on + strandat 4039.885 kb on + strandat 4039.886 kb on - strandat 4039.964 kb on + strandat 4040.029 kb on - strand, within ECOLIN_RS20125at 4040.029 kb on - strand, within ECOLIN_RS20125at 4040.029 kb on - strand, within ECOLIN_RS20125at 4040.219 kb on - strand, within ECOLIN_RS20125at 4040.219 kb on - strand, within ECOLIN_RS20125at 4040.291 kb on - strand, within ECOLIN_RS20125at 4040.399 kb on - strand, within ECOLIN_RS20125at 4040.546 kb on - strand, within ECOLIN_RS20125

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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4,037,554 + ECOLIN_RS20115 0.59 +0.4
4,037,666 - ECOLIN_RS20115 0.73 -0.0
4,038,043 + +0.0
4,038,043 + +0.0
4,038,043 + -0.2
4,038,147 - -0.3
4,038,177 + +0.0
4,038,207 - +0.7
4,038,269 - -0.2
4,038,641 - ECOLIN_RS20120 0.27 +1.5
4,039,739 + -0.0
4,039,865 + +0.3
4,039,885 + +0.1
4,039,886 - +0.2
4,039,964 + -0.3
4,040,029 - ECOLIN_RS20125 0.12 -0.6
4,040,029 - ECOLIN_RS20125 0.12 +0.3
4,040,029 - ECOLIN_RS20125 0.12 +0.2
4,040,219 - ECOLIN_RS20125 0.25 +0.5
4,040,219 - ECOLIN_RS20125 0.25 -0.3
4,040,291 - ECOLIN_RS20125 0.30 -0.0
4,040,399 - ECOLIN_RS20125 0.38 +0.4
4,040,546 - ECOLIN_RS20125 0.48 +0.2

Or see this region's nucleotide sequence