Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS07675 and ECOLIN_RS07680 are separated by 209 nucleotides ECOLIN_RS07680 and ECOLIN_RS07685 are separated by 318 nucleotides ECOLIN_RS07685 and ECOLIN_RS07690 are separated by 2 nucleotides ECOLIN_RS07690 and ECOLIN_RS31380 are separated by 113 nucleotides ECOLIN_RS31380 and ECOLIN_RS07705 are separated by 149 nucleotides
ECOLIN_RS07675: ECOLIN_RS07675 - type I DNA topoisomerase, at 1,500,847 to 1,503,444
_RS07675
ECOLIN_RS07680: ECOLIN_RS07680 - HTH-type transcriptional regulator CysB, at 1,503,654 to 1,504,628
_RS07680
ECOLIN_RS07685: ECOLIN_RS07685 - YmiA family putative membrane protein, at 1,504,947 to 1,505,087
_RS07685
ECOLIN_RS07690: ECOLIN_RS07690 - protein YciX, at 1,505,090 to 1,505,257
_RS07690
ECOLIN_RS31380: ECOLIN_RS31380 - small membrane protein YmiC, at 1,505,371 to 1,505,466
_RS31380
ECOLIN_RS07705: ECOLIN_RS07705 - aconitate hydratase AcnA, at 1,505,616 to 1,508,291
_RS07705
Position (kb) 1503
1504
1505 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1503.541 kb on + strand at 1503.939 kb on + strand, within ECOLIN_RS07680 at 1504.126 kb on + strand, within ECOLIN_RS07680 at 1504.242 kb on + strand, within ECOLIN_RS07680 at 1504.245 kb on - strand, within ECOLIN_RS07680
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 1,503,541 + -0.5 1,503,939 + ECOLIN_RS07680 0.29 +1.1 1,504,126 + ECOLIN_RS07680 0.48 -3.0 1,504,242 + ECOLIN_RS07680 0.60 -2.6 1,504,245 - ECOLIN_RS07680 0.61 -0.6
Or see this region's nucleotide sequence