Experiment: Phi92
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS00565 and ECOLIN_RS00570 overlap by 11 nucleotides ECOLIN_RS00570 and ECOLIN_RS00575 are separated by 9 nucleotides ECOLIN_RS00575 and ECOLIN_RS00585 are separated by 203 nucleotides
ECOLIN_RS00565: ECOLIN_RS00565 - protein transport protein HofC, at 117,607 to 118,809
_RS00565
ECOLIN_RS00570: ECOLIN_RS00570 - type II secretion system protein GspE, at 118,799 to 120,184
_RS00570
ECOLIN_RS00575: ECOLIN_RS00575 - prepilin peptidase-dependent pilin, at 120,194 to 120,634
_RS00575
ECOLIN_RS00585: ECOLIN_RS00585 - carboxylating nicotinate-nucleotide diphosphorylase, at 120,838 to 121,731
_RS00585
Position (kb) 118
119
120
121 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 117.892 kb on - strand, within ECOLIN_RS00565 at 118.236 kb on - strand, within ECOLIN_RS00565 at 118.236 kb on - strand, within ECOLIN_RS00565 at 118.236 kb on - strand, within ECOLIN_RS00565 at 118.436 kb on + strand, within ECOLIN_RS00565 at 118.451 kb on - strand, within ECOLIN_RS00565 at 118.468 kb on + strand, within ECOLIN_RS00565 at 118.951 kb on + strand, within ECOLIN_RS00570 at 118.961 kb on + strand, within ECOLIN_RS00570 at 119.069 kb on + strand, within ECOLIN_RS00570 at 119.069 kb on + strand, within ECOLIN_RS00570 at 120.179 kb on - strand at 120.195 kb on + strand at 120.269 kb on - strand, within ECOLIN_RS00575 at 120.269 kb on - strand, within ECOLIN_RS00575 at 120.447 kb on - strand, within ECOLIN_RS00575 at 120.447 kb on - strand, within ECOLIN_RS00575 at 120.507 kb on - strand, within ECOLIN_RS00575 at 120.507 kb on - strand, within ECOLIN_RS00575 at 120.507 kb on - strand, within ECOLIN_RS00575 at 120.538 kb on - strand, within ECOLIN_RS00575 at 120.901 kb on - strand at 121.096 kb on + strand, within ECOLIN_RS00585
Per-strain Table
Position Strand Gene LocusTag Fraction Phi92 remove 117,892 - ECOLIN_RS00565 0.24 +0.4 118,236 - ECOLIN_RS00565 0.52 +0.7 118,236 - ECOLIN_RS00565 0.52 +0.1 118,236 - ECOLIN_RS00565 0.52 +1.4 118,436 + ECOLIN_RS00565 0.69 -0.3 118,451 - ECOLIN_RS00565 0.70 -0.6 118,468 + ECOLIN_RS00565 0.72 +0.8 118,951 + ECOLIN_RS00570 0.11 +0.0 118,961 + ECOLIN_RS00570 0.12 -0.5 119,069 + ECOLIN_RS00570 0.19 -3.5 119,069 + ECOLIN_RS00570 0.19 -1.4 120,179 - +0.8 120,195 + -0.0 120,269 - ECOLIN_RS00575 0.17 +5.0 120,269 - ECOLIN_RS00575 0.17 -0.1 120,447 - ECOLIN_RS00575 0.57 -0.2 120,447 - ECOLIN_RS00575 0.57 -0.7 120,507 - ECOLIN_RS00575 0.71 +0.2 120,507 - ECOLIN_RS00575 0.71 +0.8 120,507 - ECOLIN_RS00575 0.71 +0.2 120,538 - ECOLIN_RS00575 0.78 +0.6 120,901 - +0.6 121,096 + ECOLIN_RS00585 0.29 +0.3
Or see this region's nucleotide sequence