Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02165

Experiment: Phi92

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02160 and ECOLIN_RS02165 are separated by 39 nucleotidesECOLIN_RS02165 and ECOLIN_RS02170 are separated by 324 nucleotides ECOLIN_RS02160: ECOLIN_RS02160 - 2-methylcitrate dehydratase, at 439,705 to 441,156 _RS02160 ECOLIN_RS02165: ECOLIN_RS02165 - propionate--CoA ligase, at 441,196 to 443,082 _RS02165 ECOLIN_RS02170: ECOLIN_RS02170 - cytosine permease, at 443,407 to 444,666 _RS02170 Position (kb) 441 442 443 444Strain fitness (log2 ratio) -1 0 1at 440.196 kb on + strand, within ECOLIN_RS02160at 440.196 kb on + strand, within ECOLIN_RS02160at 440.197 kb on - strand, within ECOLIN_RS02160at 441.134 kb on - strandat 441.145 kb on + strandat 441.441 kb on + strand, within ECOLIN_RS02165at 442.452 kb on + strand, within ECOLIN_RS02165at 442.662 kb on + strand, within ECOLIN_RS02165at 443.080 kb on + strandat 443.306 kb on - strandat 443.315 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phi92
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440,196 + ECOLIN_RS02160 0.34 +0.8
440,196 + ECOLIN_RS02160 0.34 -0.3
440,197 - ECOLIN_RS02160 0.34 +0.7
441,134 - -0.1
441,145 + -0.4
441,441 + ECOLIN_RS02165 0.13 +1.5
442,452 + ECOLIN_RS02165 0.67 +0.4
442,662 + ECOLIN_RS02165 0.78 -0.6
443,080 + -0.2
443,306 - +0.4
443,315 - +0.0

Or see this region's nucleotide sequence