Experiment: Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS01850 and ECOLIN_RS30360 are separated by 592 nucleotides ECOLIN_RS30360 and ECOLIN_RS32140 are separated by 216 nucleotides ECOLIN_RS32140 and ECOLIN_RS32255 are separated by 48 nucleotides ECOLIN_RS32255 and ECOLIN_RS01880 are separated by 262 nucleotides
ECOLIN_RS01850: ECOLIN_RS01850 - vacuolating autotransporter toxin Vat, at 370,350 to 374,480
_RS01850
ECOLIN_RS30360: ECOLIN_RS30360 - IS1 family transposase, at 375,073 to 375,766
_RS30360
ECOLIN_RS32140: ECOLIN_RS32140 - integrase, at 375,983 to 376,141
_RS32140
ECOLIN_RS32255: ECOLIN_RS32255 - hypothetical protein, at 376,190 to 376,327
_RS32255
ECOLIN_RS01880: ECOLIN_RS01880 - YagU family protein, at 376,590 to 377,204
_RS01880
Position (kb) 375
376 Strain fitness (log2 ratio) -1
0
1 at 374.088 kb on - strand at 374.098 kb on - strand at 374.152 kb on - strand at 374.282 kb on - strand at 374.442 kb on - strand at 374.450 kb on - strand at 374.450 kb on - strand at 375.135 kb on + strand at 375.196 kb on + strand, within ECOLIN_RS30360 at 375.643 kb on + strand, within ECOLIN_RS30360 at 376.055 kb on + strand, within ECOLIN_RS32140 at 376.562 kb on - strand at 376.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas31 remove 374,088 - +0.4 374,098 - +1.3 374,152 - -1.3 374,282 - +0.1 374,442 - -1.7 374,450 - +0.2 374,450 - +0.9 375,135 + +0.0 375,196 + ECOLIN_RS30360 0.18 +0.0 375,643 + ECOLIN_RS30360 0.82 +0.3 376,055 + ECOLIN_RS32140 0.45 -0.8 376,562 - +0.1 376,597 + +1.0
Or see this region's nucleotide sequence