Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS25075

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS25060 and ECOLIN_RS25065 are separated by 140 nucleotidesECOLIN_RS25065 and ECOLIN_RS25070 are separated by 38 nucleotidesECOLIN_RS25070 and ECOLIN_RS25075 are separated by 34 nucleotidesECOLIN_RS25075 and ECOLIN_RS25080 are separated by 28 nucleotidesECOLIN_RS25080 and ECOLIN_RS25090 are separated by 290 nucleotides ECOLIN_RS25060: ECOLIN_RS25060 - siderophore-iron reductase FhuF, at 5,041,598 to 5,042,386 _RS25060 ECOLIN_RS25065: ECOLIN_RS25065 - DUF1435 domain-containing protein, at 5,042,527 to 5,042,763 _RS25065 ECOLIN_RS25070: ECOLIN_RS25070 - tRNA-Leu, at 5,042,802 to 5,042,888 _RS25070 ECOLIN_RS25075: ECOLIN_RS25075 - tRNA-Leu, at 5,042,923 to 5,043,009 _RS25075 ECOLIN_RS25080: ECOLIN_RS25080 - tRNA-Leu, at 5,043,038 to 5,043,124 _RS25080 ECOLIN_RS25090: ECOLIN_RS25090 - 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC, at 5,043,415 to 5,044,446 _RS25090 Position (kb) 5042 5043 5044Strain fitness (log2 ratio) -1 0 1at 5042.900 kb on - strandat 5043.198 kb on + strandat 5043.216 kb on + strandat 5043.263 kb on - strandat 5043.339 kb on - strandat 5043.370 kb on + strandat 5043.371 kb on - strandat 5043.542 kb on - strand, within ECOLIN_RS25090at 5043.567 kb on + strand, within ECOLIN_RS25090at 5043.883 kb on + strand, within ECOLIN_RS25090at 5043.992 kb on - strand, within ECOLIN_RS25090

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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5,042,900 - +0.3
5,043,198 + +0.3
5,043,216 + +0.9
5,043,263 - -0.2
5,043,339 - +0.0
5,043,370 + +0.3
5,043,371 - +0.4
5,043,542 - ECOLIN_RS25090 0.12 -0.0
5,043,567 + ECOLIN_RS25090 0.15 +0.2
5,043,883 + ECOLIN_RS25090 0.45 -0.3
5,043,992 - ECOLIN_RS25090 0.56 -0.0

Or see this region's nucleotide sequence