Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17420

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17410 and ECOLIN_RS17415 are separated by 108 nucleotidesECOLIN_RS17415 and ECOLIN_RS17420 are separated by 52 nucleotidesECOLIN_RS17420 and ECOLIN_RS17425 are separated by 151 nucleotidesECOLIN_RS17425 and ECOLIN_RS17430 overlap by 4 nucleotides ECOLIN_RS17410: ECOLIN_RS17410 - ABC transporter substrate-binding protein, at 3,509,187 to 3,510,794 _RS17410 ECOLIN_RS17415: ECOLIN_RS17415 - GyrI-like domain-containing protein, at 3,510,903 to 3,511,385 _RS17415 ECOLIN_RS17420: ECOLIN_RS17420 - OB fold stress tolerance protein YgiW, at 3,511,438 to 3,511,830 _RS17420 ECOLIN_RS17425: ECOLIN_RS17425 - quorum sensing response regulator transcription factor QseB, at 3,511,982 to 3,512,641 _RS17425 ECOLIN_RS17430: ECOLIN_RS17430 - quorum sensing histidine kinase QseC, at 3,512,638 to 3,513,987 _RS17430 Position (kb) 3511 3512Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3510.516 kb on - strand, within ECOLIN_RS17410at 3510.538 kb on - strand, within ECOLIN_RS17410at 3510.592 kb on - strand, within ECOLIN_RS17410at 3510.790 kb on + strandat 3510.790 kb on + strandat 3510.790 kb on + strandat 3510.790 kb on + strandat 3510.919 kb on + strandat 3510.919 kb on + strandat 3510.919 kb on + strandat 3510.919 kb on + strandat 3510.920 kb on - strandat 3510.920 kb on - strandat 3510.920 kb on - strandat 3510.920 kb on - strandat 3511.166 kb on + strand, within ECOLIN_RS17415at 3511.291 kb on + strand, within ECOLIN_RS17415at 3511.358 kb on + strandat 3511.358 kb on + strandat 3511.358 kb on + strandat 3511.440 kb on - strandat 3511.447 kb on + strandat 3511.448 kb on - strandat 3511.522 kb on - strand, within ECOLIN_RS17420at 3511.578 kb on + strand, within ECOLIN_RS17420at 3511.604 kb on + strand, within ECOLIN_RS17420at 3511.604 kb on + strand, within ECOLIN_RS17420at 3511.605 kb on - strand, within ECOLIN_RS17420at 3511.605 kb on - strand, within ECOLIN_RS17420at 3511.605 kb on - strand, within ECOLIN_RS17420at 3511.684 kb on + strand, within ECOLIN_RS17420at 3511.684 kb on + strand, within ECOLIN_RS17420at 3511.684 kb on + strand, within ECOLIN_RS17420at 3511.685 kb on - strand, within ECOLIN_RS17420at 3511.785 kb on + strand, within ECOLIN_RS17420at 3511.834 kb on + strandat 3511.922 kb on - strandat 3511.959 kb on - strandat 3511.998 kb on + strandat 3512.102 kb on + strand, within ECOLIN_RS17425at 3512.102 kb on + strand, within ECOLIN_RS17425at 3512.102 kb on + strand, within ECOLIN_RS17425at 3512.115 kb on - strand, within ECOLIN_RS17425at 3512.298 kb on + strand, within ECOLIN_RS17425at 3512.460 kb on + strand, within ECOLIN_RS17425at 3512.516 kb on + strand, within ECOLIN_RS17425at 3512.516 kb on + strand, within ECOLIN_RS17425at 3512.517 kb on - strand, within ECOLIN_RS17425at 3512.517 kb on - strand, within ECOLIN_RS17425at 3512.518 kb on + strand, within ECOLIN_RS17425at 3512.519 kb on - strand, within ECOLIN_RS17425at 3512.629 kb on - strandat 3512.658 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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3,510,516 - ECOLIN_RS17410 0.83 +0.5
3,510,538 - ECOLIN_RS17410 0.84 -0.2
3,510,592 - ECOLIN_RS17410 0.87 +0.2
3,510,790 + +0.4
3,510,790 + +3.3
3,510,790 + +1.1
3,510,790 + +0.4
3,510,919 + +0.1
3,510,919 + -0.1
3,510,919 + +0.3
3,510,919 + -0.1
3,510,920 - +0.3
3,510,920 - +0.5
3,510,920 - +0.0
3,510,920 - +0.5
3,511,166 + ECOLIN_RS17415 0.54 -4.3
3,511,291 + ECOLIN_RS17415 0.80 +0.6
3,511,358 + +0.7
3,511,358 + +0.4
3,511,358 + +0.3
3,511,440 - -0.0
3,511,447 + -0.2
3,511,448 - +0.1
3,511,522 - ECOLIN_RS17420 0.21 +0.2
3,511,578 + ECOLIN_RS17420 0.36 +0.4
3,511,604 + ECOLIN_RS17420 0.42 +0.5
3,511,604 + ECOLIN_RS17420 0.42 +0.1
3,511,605 - ECOLIN_RS17420 0.42 +1.0
3,511,605 - ECOLIN_RS17420 0.42 +0.6
3,511,605 - ECOLIN_RS17420 0.42 +0.8
3,511,684 + ECOLIN_RS17420 0.63 -0.3
3,511,684 + ECOLIN_RS17420 0.63 -0.7
3,511,684 + ECOLIN_RS17420 0.63 -1.1
3,511,685 - ECOLIN_RS17420 0.63 -0.1
3,511,785 + ECOLIN_RS17420 0.88 -0.7
3,511,834 + -0.4
3,511,922 - +0.3
3,511,959 - -0.3
3,511,998 + +0.5
3,512,102 + ECOLIN_RS17425 0.18 +0.9
3,512,102 + ECOLIN_RS17425 0.18 -0.0
3,512,102 + ECOLIN_RS17425 0.18 +0.2
3,512,115 - ECOLIN_RS17425 0.20 -0.3
3,512,298 + ECOLIN_RS17425 0.48 +0.4
3,512,460 + ECOLIN_RS17425 0.72 -0.1
3,512,516 + ECOLIN_RS17425 0.81 +0.2
3,512,516 + ECOLIN_RS17425 0.81 -0.3
3,512,517 - ECOLIN_RS17425 0.81 +0.5
3,512,517 - ECOLIN_RS17425 0.81 -0.2
3,512,518 + ECOLIN_RS17425 0.81 +0.1
3,512,519 - ECOLIN_RS17425 0.81 +1.4
3,512,629 - +0.3
3,512,658 + -2.6

Or see this region's nucleotide sequence