Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17005

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17000 and ECOLIN_RS17005 are separated by 71 nucleotidesECOLIN_RS17005 and ECOLIN_RS17010 are separated by 23 nucleotides ECOLIN_RS17000: ECOLIN_RS17000 - KpsF/GutQ family sugar-phosphate isomerase, at 3,420,771 to 3,421,754 _RS17000 ECOLIN_RS17005: ECOLIN_RS17005 - capsule polysaccharide export inner-membrane protein KpsE, at 3,421,826 to 3,422,974 _RS17005 ECOLIN_RS17010: ECOLIN_RS17010 - polysaccharide biosynthesis/export family protein, at 3,422,998 to 3,424,674 _RS17010 Position (kb) 3421 3422 3423Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 3420.935 kb on + strand, within ECOLIN_RS17000at 3421.384 kb on + strand, within ECOLIN_RS17000at 3421.752 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.756 kb on + strandat 3421.757 kb on - strandat 3421.823 kb on + strandat 3421.982 kb on - strand, within ECOLIN_RS17005at 3422.022 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.536 kb on + strand, within ECOLIN_RS17005at 3422.591 kb on + strand, within ECOLIN_RS17005at 3423.066 kb on + strandat 3423.268 kb on + strand, within ECOLIN_RS17010at 3423.268 kb on + strand, within ECOLIN_RS17010at 3423.446 kb on + strand, within ECOLIN_RS17010at 3423.525 kb on + strand, within ECOLIN_RS17010at 3423.747 kb on + strand, within ECOLIN_RS17010

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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3,420,935 + ECOLIN_RS17000 0.17 -4.3
3,421,384 + ECOLIN_RS17000 0.62 -2.5
3,421,752 + -0.5
3,421,756 + -2.0
3,421,756 + -2.2
3,421,756 + -1.9
3,421,756 + -2.3
3,421,756 + -1.4
3,421,757 - -7.9
3,421,823 + -4.1
3,421,982 - ECOLIN_RS17005 0.14 -4.2
3,422,022 + ECOLIN_RS17005 0.17 -8.2
3,422,261 + ECOLIN_RS17005 0.38 -4.0
3,422,261 + ECOLIN_RS17005 0.38 -4.5
3,422,536 + ECOLIN_RS17005 0.62 +0.6
3,422,591 + ECOLIN_RS17005 0.67 -4.1
3,423,066 + -8.3
3,423,268 + ECOLIN_RS17010 0.16 -6.5
3,423,268 + ECOLIN_RS17010 0.16 -5.4
3,423,446 + ECOLIN_RS17010 0.27 -4.8
3,423,525 + ECOLIN_RS17010 0.31 -5.6
3,423,747 + ECOLIN_RS17010 0.45 -6.1

Or see this region's nucleotide sequence