Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14680

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14675 and ECOLIN_RS14680 are separated by 134 nucleotidesECOLIN_RS14680 and ECOLIN_RS14690 are separated by 269 nucleotidesECOLIN_RS14690 and ECOLIN_RS31555 are separated by 103 nucleotidesECOLIN_RS31555 and ECOLIN_RS14695 are separated by 98 nucleotides ECOLIN_RS14675: ECOLIN_RS14675 - 23S rRNA pseudouridine(1911/1915/1917) synthase RluD, at 2,971,593 to 2,972,573 _RS14675 ECOLIN_RS14680: ECOLIN_RS14680 - outer membrane protein assembly factor BamD, at 2,972,708 to 2,973,445 _RS14680 ECOLIN_RS14690: ECOLIN_RS14690 - ribosome-associated translation inhibitor RaiA, at 2,973,715 to 2,974,056 _RS14690 ECOLIN_RS31555: ECOLIN_RS31555 - pheA operon leader peptide PheL, at 2,974,160 to 2,974,207 _RS31555 ECOLIN_RS14695: ECOLIN_RS14695 - bifunctional chorismate mutase/prephenate dehydratase, at 2,974,306 to 2,975,466 _RS14695 Position (kb) 2972 2973 2974Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2971.758 kb on - strand, within ECOLIN_RS14675at 2971.816 kb on + strand, within ECOLIN_RS14675at 2971.877 kb on + strand, within ECOLIN_RS14675at 2971.878 kb on - strand, within ECOLIN_RS14675at 2971.958 kb on + strand, within ECOLIN_RS14675at 2972.019 kb on + strand, within ECOLIN_RS14675at 2972.040 kb on - strand, within ECOLIN_RS14675at 2972.263 kb on - strand, within ECOLIN_RS14675at 2972.263 kb on - strand, within ECOLIN_RS14675at 2972.515 kb on - strandat 2972.573 kb on + strandat 2972.574 kb on - strandat 2972.574 kb on - strandat 2972.574 kb on - strandat 2972.574 kb on - strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.575 kb on + strandat 2972.576 kb on - strandat 2972.576 kb on - strandat 2972.576 kb on - strandat 2972.576 kb on - strandat 2972.576 kb on - strandat 2972.607 kb on - strandat 2972.607 kb on - strandat 2972.607 kb on - strandat 2972.621 kb on + strandat 2972.626 kb on + strandat 2972.626 kb on + strandat 2972.627 kb on - strandat 2973.443 kb on + strandat 2973.443 kb on + strandat 2973.444 kb on - strandat 2973.500 kb on - strandat 2973.673 kb on + strandat 2974.062 kb on + strandat 2974.134 kb on + strandat 2974.195 kb on + strand, within ECOLIN_RS31555at 2974.253 kb on + strandat 2974.254 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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2,971,758 - ECOLIN_RS14675 0.17 -1.4
2,971,816 + ECOLIN_RS14675 0.23 -2.1
2,971,877 + ECOLIN_RS14675 0.29 -1.6
2,971,878 - ECOLIN_RS14675 0.29 -1.2
2,971,958 + ECOLIN_RS14675 0.37 -2.5
2,972,019 + ECOLIN_RS14675 0.43 -1.8
2,972,040 - ECOLIN_RS14675 0.46 -2.0
2,972,263 - ECOLIN_RS14675 0.68 -1.6
2,972,263 - ECOLIN_RS14675 0.68 -1.3
2,972,515 - -2.1
2,972,573 + -1.2
2,972,574 - -1.6
2,972,574 - -2.0
2,972,574 - -1.4
2,972,574 - -1.6
2,972,575 + -3.5
2,972,575 + -2.6
2,972,575 + -1.8
2,972,575 + -1.7
2,972,575 + -1.7
2,972,575 + -1.2
2,972,575 + -2.6
2,972,575 + -2.3
2,972,575 + -1.4
2,972,576 - -1.8
2,972,576 - -1.7
2,972,576 - -2.0
2,972,576 - -3.4
2,972,576 - -1.6
2,972,607 - +0.7
2,972,607 - +0.9
2,972,607 - +2.8
2,972,621 + -0.8
2,972,626 + -2.5
2,972,626 + -1.9
2,972,627 - -0.2
2,973,443 + +1.1
2,973,443 + +1.2
2,973,444 - +1.1
2,973,500 - +0.4
2,973,673 + +0.3
2,974,062 + +0.1
2,974,134 + -0.1
2,974,195 + ECOLIN_RS31555 0.73 +0.4
2,974,253 + -0.2
2,974,254 - +0.1

Or see this region's nucleotide sequence