Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12185

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12180 and ECOLIN_RS12185 overlap by 4 nucleotidesECOLIN_RS12185 and ECOLIN_RS12190 overlap by 4 nucleotidesECOLIN_RS12190 and ECOLIN_RS31520 are separated by 217 nucleotides ECOLIN_RS12180: ECOLIN_RS12180 - protein kinase YegI, at 2,409,543 to 2,411,483 _RS12180 ECOLIN_RS12185: ECOLIN_RS12185 - protein-serine/threonine phosphatase PphC, at 2,411,480 to 2,412,241 _RS12185 ECOLIN_RS12190: ECOLIN_RS12190 - VWA domain-containing protein, at 2,412,238 to 2,412,897 _RS12190 ECOLIN_RS31520: ECOLIN_RS31520 - type I toxin-antitoxin system Ibs family toxin, at 2,413,115 to 2,413,171 _RS31520 Position (kb) 2411 2412 2413Strain fitness (log2 ratio) -1 0 1at 2410.877 kb on + strand, within ECOLIN_RS12180at 2411.182 kb on + strand, within ECOLIN_RS12180at 2411.183 kb on - strand, within ECOLIN_RS12180at 2412.040 kb on - strand, within ECOLIN_RS12185at 2412.920 kb on + strandat 2413.116 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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2,410,877 + ECOLIN_RS12180 0.69 -0.2
2,411,182 + ECOLIN_RS12180 0.84 -0.1
2,411,183 - ECOLIN_RS12180 0.84 -0.2
2,412,040 - ECOLIN_RS12185 0.73 +0.1
2,412,920 + +0.4
2,413,116 + -0.4

Or see this region's nucleotide sequence