Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS24275

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS24265 and ECOLIN_RS24270 are separated by 90 nucleotidesECOLIN_RS24270 and ECOLIN_RS24275 are separated by 126 nucleotidesECOLIN_RS24275 and ECOLIN_RS24280 are separated by 18 nucleotidesECOLIN_RS24280 and ECOLIN_RS24285 are separated by 49 nucleotides ECOLIN_RS24265: ECOLIN_RS24265 - ribonuclease R, at 4,879,672 to 4,882,113 _RS24265 ECOLIN_RS24270: ECOLIN_RS24270 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 4,882,204 to 4,882,935 _RS24270 ECOLIN_RS24275: ECOLIN_RS24275 - DUF2170 family protein, at 4,883,062 to 4,883,463 _RS24275 ECOLIN_RS24280: ECOLIN_RS24280 - PspA/IM30 family protein, at 4,883,482 to 4,884,180 _RS24280 ECOLIN_RS24285: ECOLIN_RS24285 - YjfK family protein, at 4,884,230 to 4,884,889 _RS24285 Position (kb) 4883 4884Strain fitness (log2 ratio) -2 -1 0 1 2at 4882.203 kb on - strandat 4882.511 kb on + strand, within ECOLIN_RS24270at 4882.689 kb on + strand, within ECOLIN_RS24270at 4882.750 kb on + strand, within ECOLIN_RS24270at 4882.933 kb on + strandat 4882.989 kb on - strandat 4883.505 kb on + strandat 4883.505 kb on + strandat 4884.127 kb on - strandat 4884.146 kb on + strandat 4884.147 kb on - strandat 4884.213 kb on + strandat 4884.214 kb on - strandat 4884.214 kb on - strandat 4884.229 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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4,882,203 - -0.4
4,882,511 + ECOLIN_RS24270 0.42 -1.0
4,882,689 + ECOLIN_RS24270 0.66 -2.0
4,882,750 + ECOLIN_RS24270 0.75 -1.8
4,882,933 + -0.1
4,882,989 - +2.2
4,883,505 + -0.1
4,883,505 + -0.3
4,884,127 - +0.3
4,884,146 + +0.7
4,884,147 - +0.7
4,884,213 + +0.2
4,884,214 - +0.1
4,884,214 - +0.1
4,884,229 + -0.1

Or see this region's nucleotide sequence