Experiment: Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS20665 and ECOLIN_RS20670 are separated by 81 nucleotides ECOLIN_RS20670 and ECOLIN_RS20675 are separated by 25 nucleotides ECOLIN_RS20675 and ECOLIN_RS20680 are separated by 9 nucleotides
ECOLIN_RS20665: ECOLIN_RS20665 - glycosyltransferase family A protein, at 4,164,624 to 4,165,607
_RS20665
ECOLIN_RS20670: ECOLIN_RS20670 - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 4,165,689 to 4,166,717
_RS20670
ECOLIN_RS20675: ECOLIN_RS20675 - lipopolysaccharide core heptose(II) kinase RfaY, at 4,166,743 to 4,167,435
_RS20675
ECOLIN_RS20680: ECOLIN_RS20680 - glycosyltransferase, at 4,167,445 to 4,168,440
_RS20680
Position (kb) 4165
4166
4167 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4164.981 kb on - strand, within ECOLIN_RS20665 at 4165.135 kb on - strand, within ECOLIN_RS20665 at 4165.314 kb on + strand, within ECOLIN_RS20665 at 4166.086 kb on + strand, within ECOLIN_RS20670 at 4166.709 kb on + strand at 4167.194 kb on + strand, within ECOLIN_RS20675 at 4167.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas31 remove 4,164,981 - ECOLIN_RS20665 0.36 -3.2 4,165,135 - ECOLIN_RS20665 0.52 -3.1 4,165,314 + ECOLIN_RS20665 0.70 -2.6 4,166,086 + ECOLIN_RS20670 0.39 -2.4 4,166,709 + -1.7 4,167,194 + ECOLIN_RS20675 0.65 -4.0 4,167,422 - -2.7
Or see this region's nucleotide sequence