Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17660

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17650 and ECOLIN_RS17655 are separated by 79 nucleotidesECOLIN_RS17655 and ECOLIN_RS17660 are separated by 90 nucleotidesECOLIN_RS17660 and ECOLIN_RS17665 are separated by 104 nucleotidesECOLIN_RS17665 and ECOLIN_RS17670 are separated by 12 nucleotides ECOLIN_RS17650: ECOLIN_RS17650 - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 3,556,331 to 3,557,569 _RS17650 ECOLIN_RS17655: ECOLIN_RS17655 - undecaprenyl-diphosphate phosphatase, at 3,557,649 to 3,558,470 _RS17655 ECOLIN_RS17660: ECOLIN_RS17660 - bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase, at 3,558,561 to 3,558,929 _RS17660 ECOLIN_RS17665: ECOLIN_RS17665 - glycerol-3-phosphate 1-O-acyltransferase PlsY, at 3,559,034 to 3,559,651 _RS17665 ECOLIN_RS17670: ECOLIN_RS17670 - L-tartrate utilization transcriptional activator TtdR, at 3,559,664 to 3,560,596 _RS17670 Position (kb) 3558 3559Strain fitness (log2 ratio) -1 0 1at 3557.630 kb on - strandat 3557.640 kb on - strandat 3557.666 kb on - strandat 3557.666 kb on - strandat 3557.666 kb on - strandat 3557.685 kb on - strandat 3557.745 kb on - strand, within ECOLIN_RS17655at 3558.018 kb on - strand, within ECOLIN_RS17655at 3558.101 kb on - strand, within ECOLIN_RS17655at 3558.289 kb on - strand, within ECOLIN_RS17655at 3558.289 kb on - strand, within ECOLIN_RS17655at 3558.533 kb on - strandat 3558.535 kb on - strandat 3559.034 kb on - strandat 3559.034 kb on - strandat 3559.173 kb on + strand, within ECOLIN_RS17665at 3559.174 kb on - strand, within ECOLIN_RS17665at 3559.260 kb on + strand, within ECOLIN_RS17665at 3559.287 kb on + strand, within ECOLIN_RS17665at 3559.288 kb on - strand, within ECOLIN_RS17665at 3559.787 kb on - strand, within ECOLIN_RS17670

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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3,557,630 - +1.2
3,557,640 - +0.5
3,557,666 - +0.3
3,557,666 - +0.4
3,557,666 - +0.6
3,557,685 - +0.5
3,557,745 - ECOLIN_RS17655 0.12 +0.8
3,558,018 - ECOLIN_RS17655 0.45 +0.1
3,558,101 - ECOLIN_RS17655 0.55 -0.4
3,558,289 - ECOLIN_RS17655 0.78 -0.0
3,558,289 - ECOLIN_RS17655 0.78 -0.1
3,558,533 - -0.2
3,558,535 - -0.1
3,559,034 - +0.8
3,559,034 - +0.7
3,559,173 + ECOLIN_RS17665 0.22 +0.1
3,559,174 - ECOLIN_RS17665 0.23 -0.2
3,559,260 + ECOLIN_RS17665 0.37 +0.3
3,559,287 + ECOLIN_RS17665 0.41 -0.2
3,559,288 - ECOLIN_RS17665 0.41 +0.3
3,559,787 - ECOLIN_RS17670 0.13 -0.1

Or see this region's nucleotide sequence