Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS10200

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10190 and ECOLIN_RS10195 are separated by 19 nucleotidesECOLIN_RS10195 and ECOLIN_RS10200 are separated by 96 nucleotidesECOLIN_RS10200 and ECOLIN_RS10205 are separated by 130 nucleotides ECOLIN_RS10190: ECOLIN_RS10190 - carboxy terminal-processing peptidase, at 1,996,753 to 1,998,801 _RS10190 ECOLIN_RS10195: ECOLIN_RS10195 - RNA chaperone ProQ, at 1,998,821 to 1,999,519 _RS10195 ECOLIN_RS10200: ECOLIN_RS10200 - L-methionine (R)-S-oxide reductase, at 1,999,616 to 2,000,117 _RS10200 ECOLIN_RS10205: ECOLIN_RS10205 - membrane integrity lipid transport subunit YebS, at 2,000,248 to 2,001,531 _RS10205 Position (kb) 1999 2000 2001Strain fitness (log2 ratio) -3 -2 -1 0 1at 1999.035 kb on - strand, within ECOLIN_RS10195at 1999.505 kb on - strandat 1999.595 kb on - strandat 2000.224 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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1,999,035 - ECOLIN_RS10195 0.31 -2.3
1,999,505 - -2.4
1,999,595 - +0.3
2,000,224 - -3.0

Or see this region's nucleotide sequence