Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04565

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04555 and ECOLIN_RS04560 are separated by 9 nucleotidesECOLIN_RS04560 and ECOLIN_RS04565 overlap by 4 nucleotidesECOLIN_RS04565 and ECOLIN_RS04570 are separated by 59 nucleotidesECOLIN_RS04570 and ECOLIN_RS04575 are separated by 40 nucleotides ECOLIN_RS04555: ECOLIN_RS04555 - putrescine ABC transporter ATP-binding subunit PotG, at 922,040 to 923,173 _RS04555 ECOLIN_RS04560: ECOLIN_RS04560 - putrescine ABC transporter permease PotH, at 923,183 to 924,127 _RS04560 ECOLIN_RS04565: ECOLIN_RS04565 - putrescine ABC transporter permease PotI, at 924,124 to 924,969 _RS04565 ECOLIN_RS04570: ECOLIN_RS04570 - YbjO family protein, at 925,029 to 925,517 _RS04570 ECOLIN_RS04575: ECOLIN_RS04575 - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 925,558 to 926,685 _RS04575 Position (kb) 924 925Strain fitness (log2 ratio) -1 0 1at 923.584 kb on - strand, within ECOLIN_RS04560at 924.999 kb on + strandat 925.522 kb on + strandat 925.894 kb on + strand, within ECOLIN_RS04575

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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923,584 - ECOLIN_RS04560 0.42 +0.0
924,999 + +0.5
925,522 + -0.1
925,894 + ECOLIN_RS04575 0.30 +0.1

Or see this region's nucleotide sequence