Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00005

Experiment: Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00005 and ECOLIN_RS00010 are separated by 105 nucleotides ECOLIN_RS00005: ECOLIN_RS00005 - thr operon leader peptide, at 190 to 255 _RS00005 ECOLIN_RS00010: ECOLIN_RS00010 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 361 to 2,823 _RS00010 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1at 0.030 kb on - strandat 0.117 kb on + strandat 0.130 kb on - strandat 0.143 kb on + strandat 0.201 kb on + strand, within ECOLIN_RS00005at 0.354 kb on - strandat 0.354 kb on - strandat 0.354 kb on - strandat 0.568 kb on - strandat 0.773 kb on + strand, within ECOLIN_RS00010at 0.859 kb on - strand, within ECOLIN_RS00010at 1.058 kb on - strand, within ECOLIN_RS00010at 1.236 kb on + strand, within ECOLIN_RS00010

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas31
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30 - +0.3
117 + -0.4
130 - -0.4
143 + -0.3
201 + ECOLIN_RS00005 0.17 -0.2
354 - -0.8
354 - -0.2
354 - -0.2
568 - -0.4
773 + ECOLIN_RS00010 0.17 -0.1
859 - ECOLIN_RS00010 0.20 +0.3
1,058 - ECOLIN_RS00010 0.28 -0.2
1,236 + ECOLIN_RS00010 0.36 -0.8

Or see this region's nucleotide sequence