Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS31260

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02370 and ECOLIN_RS02375 are separated by 71 nucleotidesECOLIN_RS02375 and ECOLIN_RS02380 are separated by 147 nucleotidesECOLIN_RS02380 and ECOLIN_RS31260 overlap by 4 nucleotidesECOLIN_RS31260 and ECOLIN_RS02385 are separated by 80 nucleotides ECOLIN_RS02370: ECOLIN_RS02370 - AroM family protein, at 482,221 to 482,898 _RS02370 ECOLIN_RS02375: ECOLIN_RS02375 - pyrimidine/purine nucleoside phosphorylase, at 482,970 to 483,254 _RS02375 ECOLIN_RS02380: ECOLIN_RS02380 - DUF2773 domain-containing protein, at 483,402 to 483,743 _RS02380 ECOLIN_RS31260: ECOLIN_RS31260 - hypothetical protein, at 483,740 to 483,820 _RS31260 ECOLIN_RS02385: ECOLIN_RS02385 - recombination-associated protein RdgC, at 483,901 to 484,812 _RS02385 Position (kb) 483 484Strain fitness (log2 ratio) -1 0 1at 482.753 kb on + strand, within ECOLIN_RS02370at 483.072 kb on + strand, within ECOLIN_RS02375at 483.133 kb on + strand, within ECOLIN_RS02375at 483.576 kb on - strand, within ECOLIN_RS02380at 484.322 kb on - strand, within ECOLIN_RS02385at 484.366 kb on - strand, within ECOLIN_RS02385at 484.402 kb on - strand, within ECOLIN_RS02385at 484.785 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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482,753 + ECOLIN_RS02370 0.78 -0.4
483,072 + ECOLIN_RS02375 0.36 +1.6
483,133 + ECOLIN_RS02375 0.57 -1.6
483,576 - ECOLIN_RS02380 0.51 +0.5
484,322 - ECOLIN_RS02385 0.46 +0.6
484,366 - ECOLIN_RS02385 0.51 +0.4
484,402 - ECOLIN_RS02385 0.55 -0.9
484,785 - +0.5

Or see this region's nucleotide sequence