Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14030

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14015 and ECOLIN_RS14020 are separated by 11 nucleotidesECOLIN_RS14020 and ECOLIN_RS14025 are separated by 106 nucleotidesECOLIN_RS14025 and ECOLIN_RS14030 are separated by 38 nucleotidesECOLIN_RS14030 and ECOLIN_RS14035 overlap by 4 nucleotidesECOLIN_RS14035 and ECOLIN_RS14040 overlap by 4 nucleotides ECOLIN_RS14015: ECOLIN_RS14015 - aldehyde dehydrogenase family protein, at 2,813,171 to 2,814,574 _RS14015 ECOLIN_RS14020: ECOLIN_RS14020 - ethanolamine utilization microcompartment protein EutN, at 2,814,586 to 2,814,873 _RS14020 ECOLIN_RS14025: ECOLIN_RS14025 - ethanolamine utilization microcompartment protein EutM, at 2,814,980 to 2,815,273 _RS14025 ECOLIN_RS14030: ECOLIN_RS14030 - phosphate acetyltransferase, at 2,815,312 to 2,816,328 _RS14030 ECOLIN_RS14035: ECOLIN_RS14035 - ethanolamine utilization cob(I)yrinic acid a,c-diamide adenosyltransferase EutT, at 2,816,325 to 2,817,128 _RS14035 ECOLIN_RS14040: ECOLIN_RS14040 - ethanolamine utilization acetate kinase EutQ, at 2,817,125 to 2,817,826 _RS14040 Position (kb) 2815 2816 2817Strain fitness (log2 ratio) -1 0 1at 2814.833 kb on - strand, within ECOLIN_RS14020at 2814.891 kb on - strandat 2814.891 kb on - strandat 2815.205 kb on - strand, within ECOLIN_RS14025at 2817.185 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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2,814,833 - ECOLIN_RS14020 0.86 -0.6
2,814,891 - -0.4
2,814,891 - -0.2
2,815,205 - ECOLIN_RS14025 0.77 +1.1
2,817,185 - +0.3

Or see this region's nucleotide sequence