Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12280

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12275 and ECOLIN_RS12280 are separated by 47 nucleotidesECOLIN_RS12280 and ECOLIN_RS12285 are separated by 3 nucleotidesECOLIN_RS12285 and ECOLIN_RS12290 are separated by 30 nucleotidesECOLIN_RS12290 and ECOLIN_RS12295 are separated by 9 nucleotides ECOLIN_RS12275: ECOLIN_RS12275 - galactitol-1-phosphate 5-dehydrogenase, at 2,435,053 to 2,436,093 _RS12275 ECOLIN_RS12280: ECOLIN_RS12280 - PTS galactitol transporter subunit IIC, at 2,436,141 to 2,437,496 _RS12280 ECOLIN_RS12285: ECOLIN_RS12285 - PTS galactitol transporter subunit IIB, at 2,437,500 to 2,437,784 _RS12285 ECOLIN_RS12290: ECOLIN_RS12290 - PTS galactitol transporter subunit IIA, at 2,437,815 to 2,438,267 _RS12290 ECOLIN_RS12295: ECOLIN_RS12295 - tagatose-bisphosphate aldolase subunit GatZ, at 2,438,277 to 2,439,539 _RS12295 Position (kb) 2436 2437 2438Strain fitness (log2 ratio) -1 0 1at 2435.225 kb on + strand, within ECOLIN_RS12275at 2435.601 kb on + strand, within ECOLIN_RS12275at 2436.876 kb on - strand, within ECOLIN_RS12280at 2436.940 kb on + strand, within ECOLIN_RS12280

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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2,435,225 + ECOLIN_RS12275 0.17 -0.1
2,435,601 + ECOLIN_RS12275 0.53 +0.1
2,436,876 - ECOLIN_RS12280 0.54 -0.5
2,436,940 + ECOLIN_RS12280 0.59 -0.1

Or see this region's nucleotide sequence