Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17010

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17005 and ECOLIN_RS17010 are separated by 23 nucleotidesECOLIN_RS17010 and ECOLIN_RS17015 are separated by 9 nucleotidesECOLIN_RS17015 and ECOLIN_RS17020 overlap by 4 nucleotides ECOLIN_RS17005: ECOLIN_RS17005 - capsule polysaccharide export inner-membrane protein KpsE, at 3,421,826 to 3,422,974 _RS17005 ECOLIN_RS17010: ECOLIN_RS17010 - polysaccharide biosynthesis/export family protein, at 3,422,998 to 3,424,674 _RS17010 ECOLIN_RS17015: ECOLIN_RS17015 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 3,424,684 to 3,425,424 _RS17015 ECOLIN_RS17020: ECOLIN_RS17020 - capsular polysaccharide biosynthesis protein, at 3,425,421 to 3,427,448 _RS17020 Position (kb) 3422 3423 3424 3425Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1 2at 3422.022 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.261 kb on + strand, within ECOLIN_RS17005at 3422.536 kb on + strand, within ECOLIN_RS17005at 3422.591 kb on + strand, within ECOLIN_RS17005at 3423.066 kb on + strandat 3423.268 kb on + strand, within ECOLIN_RS17010at 3423.268 kb on + strand, within ECOLIN_RS17010at 3423.446 kb on + strand, within ECOLIN_RS17010at 3423.525 kb on + strand, within ECOLIN_RS17010at 3423.747 kb on + strand, within ECOLIN_RS17010at 3424.617 kb on + strandat 3424.786 kb on + strand, within ECOLIN_RS17015at 3424.960 kb on + strand, within ECOLIN_RS17015at 3424.960 kb on + strand, within ECOLIN_RS17015at 3424.965 kb on + strand, within ECOLIN_RS17015at 3425.018 kb on + strand, within ECOLIN_RS17015at 3425.212 kb on + strand, within ECOLIN_RS17015at 3425.294 kb on + strand, within ECOLIN_RS17015

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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3,422,022 + ECOLIN_RS17005 0.17 -8.1
3,422,261 + ECOLIN_RS17005 0.38 -3.9
3,422,261 + ECOLIN_RS17005 0.38 -4.4
3,422,536 + ECOLIN_RS17005 0.62 +0.4
3,422,591 + ECOLIN_RS17005 0.67 -4.1
3,423,066 + -5.4
3,423,268 + ECOLIN_RS17010 0.16 -4.9
3,423,268 + ECOLIN_RS17010 0.16 -5.3
3,423,446 + ECOLIN_RS17010 0.27 -4.7
3,423,525 + ECOLIN_RS17010 0.31 -4.6
3,423,747 + ECOLIN_RS17010 0.45 -6.0
3,424,617 + -0.1
3,424,786 + ECOLIN_RS17015 0.14 -0.8
3,424,960 + ECOLIN_RS17015 0.37 +0.3
3,424,960 + ECOLIN_RS17015 0.37 -0.7
3,424,965 + ECOLIN_RS17015 0.38 +1.9
3,425,018 + ECOLIN_RS17015 0.45 +0.0
3,425,212 + ECOLIN_RS17015 0.71 +0.1
3,425,294 + ECOLIN_RS17015 0.82 +0.9

Or see this region's nucleotide sequence