Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00010

Experiment: CEV1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00005 and ECOLIN_RS00010 are separated by 105 nucleotidesECOLIN_RS00010 and ECOLIN_RS00015 are separated by 1 nucleotidesECOLIN_RS00015 and ECOLIN_RS00020 are separated by 0 nucleotides ECOLIN_RS00005: ECOLIN_RS00005 - thr operon leader peptide, at 190 to 255 _RS00005 ECOLIN_RS00010: ECOLIN_RS00010 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 361 to 2,823 _RS00010 ECOLIN_RS00015: ECOLIN_RS00015 - homoserine kinase, at 2,825 to 3,757 _RS00015 ECOLIN_RS00020: ECOLIN_RS00020 - threonine synthase, at 3,758 to 5,044 _RS00020 Position (kb) 0 1 2 3Strain fitness (log2 ratio) -2 -1 0 1at 0.030 kb on - strandat 0.117 kb on + strandat 0.130 kb on - strandat 0.143 kb on + strandat 0.201 kb on + strand, within ECOLIN_RS00005at 0.354 kb on - strandat 0.354 kb on - strandat 0.354 kb on - strandat 0.568 kb on - strandat 0.773 kb on + strand, within ECOLIN_RS00010at 0.859 kb on - strand, within ECOLIN_RS00010at 1.058 kb on - strand, within ECOLIN_RS00010at 1.236 kb on + strand, within ECOLIN_RS00010at 1.427 kb on - strand, within ECOLIN_RS00010at 1.495 kb on + strand, within ECOLIN_RS00010at 1.565 kb on + strand, within ECOLIN_RS00010at 1.586 kb on + strand, within ECOLIN_RS00010at 1.587 kb on - strand, within ECOLIN_RS00010at 1.853 kb on + strand, within ECOLIN_RS00010at 1.899 kb on - strand, within ECOLIN_RS00010at 1.970 kb on + strand, within ECOLIN_RS00010at 2.023 kb on - strand, within ECOLIN_RS00010at 2.050 kb on + strand, within ECOLIN_RS00010at 2.284 kb on + strand, within ECOLIN_RS00010at 2.284 kb on + strand, within ECOLIN_RS00010at 2.284 kb on + strand, within ECOLIN_RS00010at 2.284 kb on + strand, within ECOLIN_RS00010at 2.286 kb on + strand, within ECOLIN_RS00010at 2.287 kb on - strand, within ECOLIN_RS00010at 2.287 kb on - strand, within ECOLIN_RS00010at 2.287 kb on - strand, within ECOLIN_RS00010at 2.287 kb on - strand, within ECOLIN_RS00010at 2.300 kb on + strand, within ECOLIN_RS00010at 2.404 kb on - strand, within ECOLIN_RS00010at 2.409 kb on - strand, within ECOLIN_RS00010at 2.593 kb on + strandat 2.604 kb on + strandat 2.701 kb on + strandat 2.701 kb on + strandat 2.702 kb on - strandat 2.703 kb on + strandat 2.710 kb on + strandat 2.796 kb on + strandat 2.928 kb on + strand, within ECOLIN_RS00015at 3.021 kb on - strand, within ECOLIN_RS00015at 3.021 kb on - strand, within ECOLIN_RS00015at 3.467 kb on + strand, within ECOLIN_RS00015

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Per-strain Table

Position Strand Gene LocusTag Fraction CEV1
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30 - -0.4
117 + -0.3
130 - +0.4
143 + +0.7
201 + ECOLIN_RS00005 0.17 +0.5
354 - -0.2
354 - +0.1
354 - +0.0
568 - -0.3
773 + ECOLIN_RS00010 0.17 +1.2
859 - ECOLIN_RS00010 0.20 -0.2
1,058 - ECOLIN_RS00010 0.28 -0.4
1,236 + ECOLIN_RS00010 0.36 -1.1
1,427 - ECOLIN_RS00010 0.43 -0.2
1,495 + ECOLIN_RS00010 0.46 -0.2
1,565 + ECOLIN_RS00010 0.49 +0.1
1,586 + ECOLIN_RS00010 0.50 +0.6
1,587 - ECOLIN_RS00010 0.50 -1.1
1,853 + ECOLIN_RS00010 0.61 -0.5
1,899 - ECOLIN_RS00010 0.62 +0.1
1,970 + ECOLIN_RS00010 0.65 -0.0
2,023 - ECOLIN_RS00010 0.67 -0.2
2,050 + ECOLIN_RS00010 0.69 +1.2
2,284 + ECOLIN_RS00010 0.78 +0.3
2,284 + ECOLIN_RS00010 0.78 -1.1
2,284 + ECOLIN_RS00010 0.78 -0.5
2,284 + ECOLIN_RS00010 0.78 +0.5
2,286 + ECOLIN_RS00010 0.78 -0.2
2,287 - ECOLIN_RS00010 0.78 -0.5
2,287 - ECOLIN_RS00010 0.78 +0.2
2,287 - ECOLIN_RS00010 0.78 -1.3
2,287 - ECOLIN_RS00010 0.78 -0.1
2,300 + ECOLIN_RS00010 0.79 -0.3
2,404 - ECOLIN_RS00010 0.83 -0.1
2,409 - ECOLIN_RS00010 0.83 -0.2
2,593 + -0.3
2,604 + -0.1
2,701 + -0.2
2,701 + -0.7
2,702 - -0.5
2,703 + -0.0
2,710 + -0.1
2,796 + +0.1
2,928 + ECOLIN_RS00015 0.11 +0.1
3,021 - ECOLIN_RS00015 0.21 +0.1
3,021 - ECOLIN_RS00015 0.21 +1.0
3,467 + ECOLIN_RS00015 0.69 -1.8

Or see this region's nucleotide sequence