Experiment: Bas27
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS14470 and ECOLIN_RS14475 are separated by 257 nucleotides ECOLIN_RS14475 and ECOLIN_RS14480 overlap by 4 nucleotides ECOLIN_RS14480 and ECOLIN_RS14485 are separated by 57 nucleotides
ECOLIN_RS14470: ECOLIN_RS14470 - phosphoribosylformylglycinamidine synthase, at 2,926,588 to 2,930,475
_RS14470
ECOLIN_RS14475: ECOLIN_RS14475 - membrane-bound lytic murein transglycosylase MltF, at 2,930,733 to 2,932,289
_RS14475
ECOLIN_RS14480: ECOLIN_RS14480 - tRNA adenosine(34) deaminase TadA, at 2,932,286 to 2,932,789
_RS14480
ECOLIN_RS14485: ECOLIN_RS14485 - phosphatidylglycerophosphatase C, at 2,932,847 to 2,933,482
_RS14485
Position (kb)
2930
2931
2932
2933 Strain fitness (log2 ratio)
-2
-1
0
1 at 2930.138 kb on + strand at 2930.138 kb on + strand at 2930.475 kb on + strand at 2930.532 kb on - strand at 2931.064 kb on - strand, within ECOLIN_RS14475 at 2931.327 kb on + strand, within ECOLIN_RS14475 at 2931.449 kb on + strand, within ECOLIN_RS14475 at 2931.552 kb on + strand, within ECOLIN_RS14475 at 2931.604 kb on + strand, within ECOLIN_RS14475 at 2931.624 kb on + strand, within ECOLIN_RS14475 at 2931.624 kb on + strand, within ECOLIN_RS14475 at 2931.882 kb on + strand, within ECOLIN_RS14475 at 2932.807 kb on + strand at 2932.808 kb on - strand at 2932.808 kb on - strand at 2932.808 kb on - strand at 2932.823 kb on + strand at 2932.844 kb on - strand at 2933.164 kb on - strand, within ECOLIN_RS14485
Per-strain Table
Position Strand Gene LocusTag Fraction Bas27 remove 2,930,138 + +0.1 2,930,138 + -1.2 2,930,475 + +0.2 2,930,532 - -0.1 2,931,064 - ECOLIN_RS14475 0.21 +0.0 2,931,327 + ECOLIN_RS14475 0.38 +0.1 2,931,449 + ECOLIN_RS14475 0.46 +0.0 2,931,552 + ECOLIN_RS14475 0.53 +0.0 2,931,604 + ECOLIN_RS14475 0.56 +0.5 2,931,624 + ECOLIN_RS14475 0.57 +0.1 2,931,624 + ECOLIN_RS14475 0.57 -0.5 2,931,882 + ECOLIN_RS14475 0.74 +0.6 2,932,807 + -2.5 2,932,808 - +0.4 2,932,808 - -2.0 2,932,808 - +0.2 2,932,823 + -0.2 2,932,844 - +0.0 2,933,164 - ECOLIN_RS14485 0.50 +1.0
Or see this region's nucleotide sequence