Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20785

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20775 and ECOLIN_RS20780 are separated by 257 nucleotidesECOLIN_RS20780 and ECOLIN_RS20785 are separated by 54 nucleotidesECOLIN_RS20785 and ECOLIN_RS20790 are separated by 18 nucleotides ECOLIN_RS20775: ECOLIN_RS20775 - NAD-dependent DNA ligase LigB, at 4,183,823 to 4,185,505 _RS20775 ECOLIN_RS20780: ECOLIN_RS20780 - guanylate kinase, at 4,185,763 to 4,186,386 _RS20780 ECOLIN_RS20785: ECOLIN_RS20785 - DNA-directed RNA polymerase subunit omega, at 4,186,441 to 4,186,716 _RS20785 ECOLIN_RS20790: ECOLIN_RS20790 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 4,186,735 to 4,188,843 _RS20790 Position (kb) 4186 4187Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4185.475 kb on + strandat 4185.479 kb on + strandat 4185.479 kb on + strandat 4185.479 kb on + strandat 4185.480 kb on - strandat 4185.483 kb on - strandat 4185.483 kb on - strandat 4185.486 kb on - strandat 4185.497 kb on + strandat 4185.498 kb on - strandat 4185.521 kb on - strandat 4185.521 kb on - strandat 4185.525 kb on - strandat 4185.525 kb on - strandat 4185.536 kb on + strandat 4185.538 kb on + strandat 4185.538 kb on + strandat 4185.538 kb on + strandat 4185.539 kb on - strandat 4185.539 kb on - strandat 4185.539 kb on - strandat 4185.609 kb on - strandat 4185.688 kb on - strandat 4185.726 kb on + strandat 4185.726 kb on + strandat 4185.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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4,185,475 + +0.5
4,185,479 + +4.1
4,185,479 + -0.1
4,185,479 + -0.5
4,185,480 - +0.2
4,185,483 - +0.3
4,185,483 - -0.1
4,185,486 - -0.4
4,185,497 + +0.3
4,185,498 - -0.2
4,185,521 - -0.1
4,185,521 - -0.5
4,185,525 - +0.0
4,185,525 - +0.1
4,185,536 + -0.4
4,185,538 + -0.1
4,185,538 + +0.2
4,185,538 + +0.0
4,185,539 - +0.2
4,185,539 - +0.9
4,185,539 - -0.1
4,185,609 - +0.5
4,185,688 - +0.3
4,185,726 + +0.0
4,185,726 + +0.3
4,185,749 + -2.0

Or see this region's nucleotide sequence