Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00040

Experiment: Bas27

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00030 and ECOLIN_RS00035 are separated by 59 nucleotidesECOLIN_RS00035 and ECOLIN_RS00040 are separated by 69 nucleotidesECOLIN_RS00040 and ECOLIN_RS00045 are separated by 278 nucleotides ECOLIN_RS00030: ECOLIN_RS00030 - DUF2502 domain-containing protein, at 5,258 to 5,554 _RS00030 ECOLIN_RS00035: ECOLIN_RS00035 - peroxide stress protein YaaA, at 5,614 to 6,390 _RS00035 ECOLIN_RS00040: ECOLIN_RS00040 - sodium:alanine symporter family protein, at 6,460 to 7,890 _RS00040 ECOLIN_RS00045: ECOLIN_RS00045 - transaldolase, at 8,169 to 9,122 _RS00045 Position (kb) 6 7 8Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5.494 kb on + strand, within ECOLIN_RS00030at 5.783 kb on - strand, within ECOLIN_RS00035at 5.888 kb on - strand, within ECOLIN_RS00035at 5.894 kb on - strand, within ECOLIN_RS00035at 5.944 kb on - strand, within ECOLIN_RS00035at 6.047 kb on + strand, within ECOLIN_RS00035at 6.048 kb on - strand, within ECOLIN_RS00035at 6.048 kb on - strand, within ECOLIN_RS00035at 6.324 kb on + strandat 6.378 kb on + strandat 6.461 kb on + strandat 6.565 kb on + strandat 6.586 kb on - strandat 6.607 kb on + strand, within ECOLIN_RS00040at 6.607 kb on + strand, within ECOLIN_RS00040at 6.782 kb on + strand, within ECOLIN_RS00040at 6.788 kb on + strand, within ECOLIN_RS00040at 6.788 kb on + strand, within ECOLIN_RS00040at 6.789 kb on - strand, within ECOLIN_RS00040at 6.789 kb on - strand, within ECOLIN_RS00040at 6.895 kb on - strand, within ECOLIN_RS00040at 7.090 kb on - strand, within ECOLIN_RS00040at 7.110 kb on + strand, within ECOLIN_RS00040at 7.111 kb on - strand, within ECOLIN_RS00040at 7.123 kb on + strand, within ECOLIN_RS00040at 7.128 kb on + strand, within ECOLIN_RS00040at 7.129 kb on - strand, within ECOLIN_RS00040at 7.342 kb on - strand, within ECOLIN_RS00040at 7.344 kb on + strand, within ECOLIN_RS00040at 7.345 kb on - strand, within ECOLIN_RS00040at 7.494 kb on + strand, within ECOLIN_RS00040at 7.495 kb on - strand, within ECOLIN_RS00040at 7.497 kb on - strand, within ECOLIN_RS00040at 7.497 kb on - strand, within ECOLIN_RS00040at 7.591 kb on - strand, within ECOLIN_RS00040at 7.744 kb on - strand, within ECOLIN_RS00040at 7.957 kb on - strandat 7.960 kb on + strandat 7.961 kb on - strandat 7.973 kb on - strandat 7.995 kb on + strandat 7.995 kb on + strandat 7.995 kb on + strandat 7.996 kb on - strandat 7.996 kb on - strandat 7.996 kb on - strandat 8.247 kb on + strandat 8.315 kb on + strand, within ECOLIN_RS00045at 8.724 kb on + strand, within ECOLIN_RS00045at 8.725 kb on - strand, within ECOLIN_RS00045at 8.834 kb on + strand, within ECOLIN_RS00045

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas27
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5,494 + ECOLIN_RS00030 0.79 +0.0
5,783 - ECOLIN_RS00035 0.22 +0.1
5,888 - ECOLIN_RS00035 0.35 -2.7
5,894 - ECOLIN_RS00035 0.36 +0.1
5,944 - ECOLIN_RS00035 0.42 -0.0
6,047 + ECOLIN_RS00035 0.56 -0.1
6,048 - ECOLIN_RS00035 0.56 -0.6
6,048 - ECOLIN_RS00035 0.56 -0.4
6,324 + +0.3
6,378 + +0.0
6,461 + +0.2
6,565 + +0.2
6,586 - +1.5
6,607 + ECOLIN_RS00040 0.10 +0.5
6,607 + ECOLIN_RS00040 0.10 -0.7
6,782 + ECOLIN_RS00040 0.23 -0.1
6,788 + ECOLIN_RS00040 0.23 +0.7
6,788 + ECOLIN_RS00040 0.23 -1.2
6,789 - ECOLIN_RS00040 0.23 +0.5
6,789 - ECOLIN_RS00040 0.23 +0.1
6,895 - ECOLIN_RS00040 0.30 +0.0
7,090 - ECOLIN_RS00040 0.44 +0.1
7,110 + ECOLIN_RS00040 0.45 -0.1
7,111 - ECOLIN_RS00040 0.45 +0.1
7,123 + ECOLIN_RS00040 0.46 -0.5
7,128 + ECOLIN_RS00040 0.47 -5.9
7,129 - ECOLIN_RS00040 0.47 -0.1
7,342 - ECOLIN_RS00040 0.62 +0.3
7,344 + ECOLIN_RS00040 0.62 -0.1
7,345 - ECOLIN_RS00040 0.62 -0.1
7,494 + ECOLIN_RS00040 0.72 -0.7
7,495 - ECOLIN_RS00040 0.72 +1.0
7,497 - ECOLIN_RS00040 0.72 -0.0
7,497 - ECOLIN_RS00040 0.72 +0.4
7,591 - ECOLIN_RS00040 0.79 +0.7
7,744 - ECOLIN_RS00040 0.90 -0.1
7,957 - -0.2
7,960 + -0.2
7,961 - +0.7
7,973 - -0.4
7,995 + +0.6
7,995 + -0.2
7,995 + +0.2
7,996 - -0.2
7,996 - +0.0
7,996 - -0.1
8,247 + -1.7
8,315 + ECOLIN_RS00045 0.15 +0.1
8,724 + ECOLIN_RS00045 0.58 +1.2
8,725 - ECOLIN_RS00045 0.58 +0.4
8,834 + ECOLIN_RS00045 0.70 -0.2

Or see this region's nucleotide sequence