Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20625

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20620 and ECOLIN_RS20625 are separated by 3 nucleotidesECOLIN_RS20625 and ECOLIN_RS20630 overlap by 14 nucleotides ECOLIN_RS20620: ECOLIN_RS20620 - murein hydrolase activator EnvC, at 4,154,356 to 4,155,615 _RS20620 ECOLIN_RS20625: ECOLIN_RS20625 - divergent polysaccharide deacetylase family protein, at 4,155,619 to 4,156,578 _RS20625 ECOLIN_RS20630: ECOLIN_RS20630 - UDP-glucuronate:LPS(HepIII) glycosyltransferase, at 4,156,565 to 4,157,599 _RS20630 Position (kb) 4155 4156 4157Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4154.746 kb on - strand, within ECOLIN_RS20620at 4154.746 kb on - strand, within ECOLIN_RS20620at 4155.296 kb on + strand, within ECOLIN_RS20620at 4155.297 kb on - strand, within ECOLIN_RS20620at 4155.457 kb on + strand, within ECOLIN_RS20620at 4155.556 kb on + strandat 4155.783 kb on - strand, within ECOLIN_RS20625at 4155.826 kb on - strand, within ECOLIN_RS20625at 4155.873 kb on + strand, within ECOLIN_RS20625at 4156.051 kb on + strand, within ECOLIN_RS20625at 4156.061 kb on - strand, within ECOLIN_RS20625at 4156.077 kb on - strand, within ECOLIN_RS20625at 4156.271 kb on + strand, within ECOLIN_RS20625at 4156.453 kb on - strand, within ECOLIN_RS20625at 4156.469 kb on + strand, within ECOLIN_RS20625at 4156.592 kb on - strandat 4156.701 kb on + strand, within ECOLIN_RS20630at 4156.702 kb on - strand, within ECOLIN_RS20630at 4156.715 kb on - strand, within ECOLIN_RS20630at 4156.747 kb on + strand, within ECOLIN_RS20630at 4157.075 kb on - strand, within ECOLIN_RS20630at 4157.162 kb on + strand, within ECOLIN_RS20630at 4157.293 kb on - strand, within ECOLIN_RS20630at 4157.293 kb on - strand, within ECOLIN_RS20630at 4157.517 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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4,154,746 - ECOLIN_RS20620 0.31 -3.3
4,154,746 - ECOLIN_RS20620 0.31 -2.9
4,155,296 + ECOLIN_RS20620 0.75 -2.9
4,155,297 - ECOLIN_RS20620 0.75 -2.2
4,155,457 + ECOLIN_RS20620 0.87 -5.0
4,155,556 + -3.1
4,155,783 - ECOLIN_RS20625 0.17 +0.7
4,155,826 - ECOLIN_RS20625 0.22 +0.5
4,155,873 + ECOLIN_RS20625 0.26 +0.7
4,156,051 + ECOLIN_RS20625 0.45 +1.0
4,156,061 - ECOLIN_RS20625 0.46 -0.1
4,156,077 - ECOLIN_RS20625 0.48 +0.5
4,156,271 + ECOLIN_RS20625 0.68 +0.3
4,156,453 - ECOLIN_RS20625 0.87 +0.2
4,156,469 + ECOLIN_RS20625 0.89 +0.2
4,156,592 - +0.5
4,156,701 + ECOLIN_RS20630 0.13 -0.2
4,156,702 - ECOLIN_RS20630 0.13 -0.9
4,156,715 - ECOLIN_RS20630 0.14 +0.4
4,156,747 + ECOLIN_RS20630 0.18 +0.5
4,157,075 - ECOLIN_RS20630 0.49 -0.0
4,157,162 + ECOLIN_RS20630 0.58 +0.1
4,157,293 - ECOLIN_RS20630 0.70 -1.7
4,157,293 - ECOLIN_RS20630 0.70 +0.5
4,157,517 - -0.3

Or see this region's nucleotide sequence