Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS19990

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS19985 and ECOLIN_RS19990 are separated by 202 nucleotidesECOLIN_RS19990 and ECOLIN_RS19995 are separated by 71 nucleotides ECOLIN_RS19985: ECOLIN_RS19985 - oligopeptidase A, at 4,010,431 to 4,012,473 _RS19985 ECOLIN_RS19990: ECOLIN_RS19990 - 23S rRNA (adenine(2030)-N(6))-methyltransferase, at 4,012,676 to 4,013,518 _RS19990 ECOLIN_RS19995: ECOLIN_RS19995 - glutathione-disulfide reductase, at 4,013,590 to 4,014,942 _RS19995 Position (kb) 4012 4013 4014Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4012.527 kb on - strandat 4012.536 kb on - strandat 4012.536 kb on - strandat 4012.546 kb on + strandat 4012.546 kb on + strandat 4012.547 kb on - strandat 4012.657 kb on + strandat 4012.916 kb on + strand, within ECOLIN_RS19990at 4012.929 kb on + strand, within ECOLIN_RS19990at 4012.964 kb on + strand, within ECOLIN_RS19990at 4012.995 kb on + strand, within ECOLIN_RS19990at 4012.996 kb on - strand, within ECOLIN_RS19990at 4013.226 kb on - strand, within ECOLIN_RS19990at 4013.249 kb on + strand, within ECOLIN_RS19990at 4013.287 kb on + strand, within ECOLIN_RS19990at 4013.496 kb on + strandat 4013.516 kb on + strandat 4013.517 kb on - strandat 4013.539 kb on + strandat 4013.540 kb on - strandat 4013.543 kb on - strandat 4013.543 kb on - strandat 4013.555 kb on - strandat 4013.584 kb on + strandat 4013.584 kb on + strandat 4013.809 kb on - strand, within ECOLIN_RS19995at 4014.119 kb on - strand, within ECOLIN_RS19995at 4014.480 kb on + strand, within ECOLIN_RS19995

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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4,012,527 - -0.3
4,012,536 - +0.1
4,012,536 - -0.0
4,012,546 + +0.4
4,012,546 + +0.6
4,012,547 - -0.1
4,012,657 + -0.0
4,012,916 + ECOLIN_RS19990 0.28 +0.1
4,012,929 + ECOLIN_RS19990 0.30 +0.5
4,012,964 + ECOLIN_RS19990 0.34 +0.5
4,012,995 + ECOLIN_RS19990 0.38 -0.8
4,012,996 - ECOLIN_RS19990 0.38 -0.1
4,013,226 - ECOLIN_RS19990 0.65 +0.5
4,013,249 + ECOLIN_RS19990 0.68 -0.0
4,013,287 + ECOLIN_RS19990 0.72 +0.1
4,013,496 + -0.2
4,013,516 + +0.5
4,013,517 - +0.1
4,013,539 + -0.1
4,013,540 - +0.3
4,013,543 - -1.8
4,013,543 - -0.4
4,013,555 - +1.2
4,013,584 + -0.6
4,013,584 + -0.1
4,013,809 - ECOLIN_RS19995 0.16 -4.8
4,014,119 - ECOLIN_RS19995 0.39 -2.8
4,014,480 + ECOLIN_RS19995 0.66 -1.3

Or see this region's nucleotide sequence