Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04515

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04510 and ECOLIN_RS04515 are separated by 269 nucleotidesECOLIN_RS04515 and ECOLIN_RS04520 are separated by 29 nucleotidesECOLIN_RS04520 and ECOLIN_RS04525 are separated by 159 nucleotidesECOLIN_RS04525 and ECOLIN_RS04530 overlap by 17 nucleotides ECOLIN_RS04510: ECOLIN_RS04510 - aspartate:alanine antiporter, at 915,185 to 916,870 _RS04510 ECOLIN_RS04515: ECOLIN_RS04515 - inner membrane protein YbjM, at 917,140 to 917,517 _RS04515 ECOLIN_RS04520: ECOLIN_RS04520 - glutaredoxin 1, at 917,547 to 917,804 _RS04520 ECOLIN_RS04525: ECOLIN_RS04525 - DUF1418 family protein, at 917,964 to 918,251 _RS04525 ECOLIN_RS04530: ECOLIN_RS04530 - nitroreductase NfsA, at 918,235 to 918,957 _RS04530 Position (kb) 917 918Strain fitness (log2 ratio) -3 -2 -1 0 1at 916.239 kb on + strand, within ECOLIN_RS04510at 916.542 kb on - strand, within ECOLIN_RS04510at 916.844 kb on + strandat 917.049 kb on + strandat 917.702 kb on - strand, within ECOLIN_RS04520at 917.768 kb on - strand, within ECOLIN_RS04520at 917.820 kb on - strandat 917.907 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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916,239 + ECOLIN_RS04510 0.63 +0.4
916,542 - ECOLIN_RS04510 0.80 +0.7
916,844 + -0.1
917,049 + -3.6
917,702 - ECOLIN_RS04520 0.60 -0.2
917,768 - ECOLIN_RS04520 0.86 -0.2
917,820 - -0.4
917,907 + +0.5

Or see this region's nucleotide sequence