Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16040

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16035 and ECOLIN_RS16040 are separated by 321 nucleotidesECOLIN_RS16040 and ECOLIN_RS16045 are separated by 2 nucleotides ECOLIN_RS16035: ECOLIN_RS16035 - molybdenum cofactor cytidylyltransferase, at 3,247,715 to 3,248,293 _RS16035 ECOLIN_RS16040: ECOLIN_RS16040 - putative selenate reductase subunit YgfK, at 3,248,615 to 3,251,713 _RS16040 ECOLIN_RS16045: ECOLIN_RS16045 - putative aminohydrolase SsnA, at 3,251,716 to 3,253,044 _RS16045 Position (kb) 3248 3249 3250 3251 3252Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1 2at 3247.676 kb on + strandat 3247.699 kb on - strandat 3247.733 kb on - strandat 3248.202 kb on - strand, within ECOLIN_RS16035at 3248.595 kb on + strandat 3248.595 kb on + strandat 3248.595 kb on + strandat 3248.597 kb on + strandat 3248.623 kb on + strandat 3248.720 kb on + strandat 3248.730 kb on + strandat 3249.089 kb on - strand, within ECOLIN_RS16040at 3249.121 kb on + strand, within ECOLIN_RS16040at 3249.175 kb on + strand, within ECOLIN_RS16040at 3249.175 kb on + strand, within ECOLIN_RS16040at 3249.273 kb on + strand, within ECOLIN_RS16040at 3249.338 kb on + strand, within ECOLIN_RS16040at 3249.338 kb on + strand, within ECOLIN_RS16040at 3249.390 kb on + strand, within ECOLIN_RS16040at 3249.390 kb on + strand, within ECOLIN_RS16040at 3249.580 kb on + strand, within ECOLIN_RS16040at 3249.583 kb on + strand, within ECOLIN_RS16040at 3249.583 kb on + strand, within ECOLIN_RS16040at 3249.583 kb on + strand, within ECOLIN_RS16040at 3249.614 kb on + strand, within ECOLIN_RS16040at 3249.614 kb on + strand, within ECOLIN_RS16040at 3249.614 kb on + strand, within ECOLIN_RS16040at 3249.617 kb on - strand, within ECOLIN_RS16040at 3249.763 kb on + strand, within ECOLIN_RS16040at 3249.763 kb on + strand, within ECOLIN_RS16040at 3249.763 kb on + strand, within ECOLIN_RS16040at 3249.764 kb on - strand, within ECOLIN_RS16040at 3249.764 kb on - strand, within ECOLIN_RS16040at 3249.807 kb on + strand, within ECOLIN_RS16040at 3249.808 kb on - strand, within ECOLIN_RS16040at 3249.980 kb on + strand, within ECOLIN_RS16040at 3250.117 kb on + strand, within ECOLIN_RS16040at 3250.139 kb on + strand, within ECOLIN_RS16040at 3250.160 kb on + strand, within ECOLIN_RS16040at 3250.160 kb on + strand, within ECOLIN_RS16040at 3250.161 kb on - strand, within ECOLIN_RS16040at 3250.947 kb on + strand, within ECOLIN_RS16040at 3251.191 kb on + strand, within ECOLIN_RS16040at 3251.192 kb on - strand, within ECOLIN_RS16040at 3251.318 kb on - strand, within ECOLIN_RS16040at 3251.321 kb on + strand, within ECOLIN_RS16040at 3251.647 kb on + strandat 3251.647 kb on + strandat 3251.648 kb on - strandat 3251.648 kb on - strandat 3251.687 kb on + strandat 3251.711 kb on + strandat 3251.712 kb on - strandat 3251.750 kb on + strandat 3251.750 kb on + strandat 3252.106 kb on + strand, within ECOLIN_RS16045at 3252.514 kb on + strand, within ECOLIN_RS16045at 3252.514 kb on + strand, within ECOLIN_RS16045

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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3,247,676 + -0.2
3,247,699 - -0.0
3,247,733 - -2.2
3,248,202 - ECOLIN_RS16035 0.84 +0.2
3,248,595 + -0.7
3,248,595 + -1.1
3,248,595 + -0.2
3,248,597 + +0.1
3,248,623 + -0.3
3,248,720 + +0.3
3,248,730 + -0.7
3,249,089 - ECOLIN_RS16040 0.15 +0.3
3,249,121 + ECOLIN_RS16040 0.16 +0.5
3,249,175 + ECOLIN_RS16040 0.18 +0.3
3,249,175 + ECOLIN_RS16040 0.18 +0.5
3,249,273 + ECOLIN_RS16040 0.21 -1.5
3,249,338 + ECOLIN_RS16040 0.23 +1.4
3,249,338 + ECOLIN_RS16040 0.23 +0.3
3,249,390 + ECOLIN_RS16040 0.25 +0.2
3,249,390 + ECOLIN_RS16040 0.25 +0.2
3,249,580 + ECOLIN_RS16040 0.31 +0.3
3,249,583 + ECOLIN_RS16040 0.31 +0.4
3,249,583 + ECOLIN_RS16040 0.31 -0.3
3,249,583 + ECOLIN_RS16040 0.31 +0.3
3,249,614 + ECOLIN_RS16040 0.32 +1.1
3,249,614 + ECOLIN_RS16040 0.32 -0.3
3,249,614 + ECOLIN_RS16040 0.32 -0.5
3,249,617 - ECOLIN_RS16040 0.32 -7.4
3,249,763 + ECOLIN_RS16040 0.37 +0.4
3,249,763 + ECOLIN_RS16040 0.37 +0.2
3,249,763 + ECOLIN_RS16040 0.37 +0.3
3,249,764 - ECOLIN_RS16040 0.37 -0.1
3,249,764 - ECOLIN_RS16040 0.37 +0.4
3,249,807 + ECOLIN_RS16040 0.38 +0.2
3,249,808 - ECOLIN_RS16040 0.38 -0.4
3,249,980 + ECOLIN_RS16040 0.44 -0.2
3,250,117 + ECOLIN_RS16040 0.48 -6.6
3,250,139 + ECOLIN_RS16040 0.49 -0.5
3,250,160 + ECOLIN_RS16040 0.50 -0.6
3,250,160 + ECOLIN_RS16040 0.50 +0.1
3,250,161 - ECOLIN_RS16040 0.50 -0.0
3,250,947 + ECOLIN_RS16040 0.75 +0.1
3,251,191 + ECOLIN_RS16040 0.83 -0.0
3,251,192 - ECOLIN_RS16040 0.83 +0.8
3,251,318 - ECOLIN_RS16040 0.87 +0.1
3,251,321 + ECOLIN_RS16040 0.87 -0.0
3,251,647 + +1.9
3,251,647 + +0.2
3,251,648 - +0.5
3,251,648 - +1.7
3,251,687 + -0.3
3,251,711 + -5.8
3,251,712 - -0.0
3,251,750 + -0.6
3,251,750 + +1.6
3,252,106 + ECOLIN_RS16045 0.29 -0.2
3,252,514 + ECOLIN_RS16045 0.60 +0.5
3,252,514 + ECOLIN_RS16045 0.60 +0.3

Or see this region's nucleotide sequence