Experiment: EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS06280 and ECOLIN_RS06285 are separated by 111 nucleotides ECOLIN_RS06285 and ECOLIN_RS06290 are separated by 197 nucleotides ECOLIN_RS06290 and ECOLIN_RS06295 are separated by 51 nucleotides ECOLIN_RS06295 and ECOLIN_RS06300 are separated by 80 nucleotides
ECOLIN_RS06280: ECOLIN_RS06280 - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 1,264,642 to 1,265,601
_RS06280
ECOLIN_RS06285: ECOLIN_RS06285 - 7-methyl-GTP pyrophosphatase, at 1,265,713 to 1,266,297
_RS06285
ECOLIN_RS06290: ECOLIN_RS06290 - 23S rRNA accumulation protein YceD, at 1,266,495 to 1,267,016
_RS06290
ECOLIN_RS06295: ECOLIN_RS06295 - 50S ribosomal protein L32, at 1,267,068 to 1,267,241
_RS06295
ECOLIN_RS06300: ECOLIN_RS06300 - phosphate acyltransferase PlsX, at 1,267,322 to 1,268,392
_RS06300
Position (kb) 1266
1267
1268 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1266.023 kb on - strand, within ECOLIN_RS06285 at 1266.570 kb on + strand, within ECOLIN_RS06290 at 1266.756 kb on + strand, within ECOLIN_RS06290 at 1267.010 kb on + strand at 1267.075 kb on + strand at 1267.156 kb on + strand, within ECOLIN_RS06295 at 1267.786 kb on + strand, within ECOLIN_RS06300 at 1267.786 kb on + strand, within ECOLIN_RS06300
Per-strain Table
Position Strand Gene LocusTag Fraction EV219 remove 1,266,023 - ECOLIN_RS06285 0.53 +0.1 1,266,570 + ECOLIN_RS06290 0.14 -2.9 1,266,756 + ECOLIN_RS06290 0.50 -2.4 1,267,010 + -4.0 1,267,075 + -4.2 1,267,156 + ECOLIN_RS06295 0.51 -1.9 1,267,786 + ECOLIN_RS06300 0.43 +0.8 1,267,786 + ECOLIN_RS06300 0.43 -0.3
Or see this region's nucleotide sequence