Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04090

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04085 and ECOLIN_RS04090 are separated by 40 nucleotidesECOLIN_RS04090 and ECOLIN_RS04095 are separated by 183 nucleotides ECOLIN_RS04085: ECOLIN_RS04085 - 6-phosphogluconolactonase, at 825,273 to 826,268 _RS04085 ECOLIN_RS04090: ECOLIN_RS04090 - LysR family transcriptional regulator, at 826,309 to 827,262 _RS04090 ECOLIN_RS04095: ECOLIN_RS04095 - 4-oxalomesaconate tautomerase, at 827,446 to 828,498 _RS04095 Position (kb) 826 827 828Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 825.530 kb on + strand, within ECOLIN_RS04085at 825.573 kb on + strand, within ECOLIN_RS04085at 825.573 kb on + strand, within ECOLIN_RS04085at 825.689 kb on + strand, within ECOLIN_RS04085at 825.971 kb on + strand, within ECOLIN_RS04085at 826.201 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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825,530 + ECOLIN_RS04085 0.26 -4.2
825,573 + ECOLIN_RS04085 0.30 -2.0
825,573 + ECOLIN_RS04085 0.30 -2.2
825,689 + ECOLIN_RS04085 0.42 -2.1
825,971 + ECOLIN_RS04085 0.70 -3.0
826,201 + -0.0

Or see this region's nucleotide sequence