Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03400

Experiment: EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03395 and ECOLIN_RS03400 are separated by 174 nucleotidesECOLIN_RS03400 and ECOLIN_RS03405 are separated by 53 nucleotidesECOLIN_RS03405 and ECOLIN_RS03410 are separated by 92 nucleotides ECOLIN_RS03395: ECOLIN_RS03395 - lipid IV(A) palmitoyltransferase PagP, at 702,363 to 702,923 _RS03395 ECOLIN_RS03400: ECOLIN_RS03400 - transcription antiterminator/RNA stability regulator CspE, at 703,098 to 703,307 _RS03400 ECOLIN_RS03405: ECOLIN_RS03405 - fluoride efflux transporter CrcB, at 703,361 to 703,744 _RS03405 ECOLIN_RS03410: ECOLIN_RS03410 - deaminated glutathione amidase, at 703,837 to 704,625 _RS03410 Position (kb) 703 704Strain fitness (log2 ratio) -2 -1 0 1at 702.326 kb on + strandat 703.121 kb on + strand, within ECOLIN_RS03400at 703.294 kb on + strandat 703.769 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV219
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702,326 + -2.5
703,121 + ECOLIN_RS03400 0.11 +0.7
703,294 + +0.5
703,769 + -1.2

Or see this region's nucleotide sequence