Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20440

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20425 and ECOLIN_RS20435 are separated by 151 nucleotidesECOLIN_RS20435 and ECOLIN_RS20440 are separated by 101 nucleotidesECOLIN_RS20440 and ECOLIN_RS20445 are separated by 200 nucleotides ECOLIN_RS20425: ECOLIN_RS20425 - valine--pyruvate transaminase, at 4,110,583 to 4,111,836 _RS20425 ECOLIN_RS20435: ECOLIN_RS20435 - 4Fe-4S binding protein, at 4,111,988 to 4,112,461 _RS20435 ECOLIN_RS20440: ECOLIN_RS20440 - IclR family transcriptional regulator YiaJ, at 4,112,563 to 4,113,411 _RS20440 ECOLIN_RS20445: ECOLIN_RS20445 - 3-dehydro-L-gulonate 2-dehydrogenase, at 4,113,612 to 4,114,610 _RS20445 Position (kb) 4112 4113 4114Strain fitness (log2 ratio) -3 -2 -1 0 1at 4111.740 kb on + strandat 4111.740 kb on + strandat 4111.753 kb on + strandat 4111.753 kb on + strandat 4111.753 kb on + strandat 4112.114 kb on - strand, within ECOLIN_RS20435at 4112.177 kb on - strand, within ECOLIN_RS20435at 4112.186 kb on - strand, within ECOLIN_RS20435at 4112.320 kb on + strand, within ECOLIN_RS20435at 4112.479 kb on - strandat 4112.616 kb on + strandat 4112.616 kb on + strandat 4112.616 kb on + strandat 4112.949 kb on - strand, within ECOLIN_RS20440at 4112.968 kb on + strand, within ECOLIN_RS20440at 4112.970 kb on + strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4113.012 kb on + strand, within ECOLIN_RS20440at 4113.013 kb on - strand, within ECOLIN_RS20440at 4113.013 kb on - strand, within ECOLIN_RS20440at 4113.055 kb on - strand, within ECOLIN_RS20440at 4113.058 kb on - strand, within ECOLIN_RS20440at 4113.150 kb on + strand, within ECOLIN_RS20440at 4113.151 kb on - strand, within ECOLIN_RS20440at 4113.635 kb on - strandat 4113.635 kb on - strandat 4113.978 kb on + strand, within ECOLIN_RS20445at 4114.005 kb on + strand, within ECOLIN_RS20445at 4114.006 kb on - strand, within ECOLIN_RS20445at 4114.159 kb on + strand, within ECOLIN_RS20445at 4114.160 kb on - strand, within ECOLIN_RS20445at 4114.195 kb on + strand, within ECOLIN_RS20445at 4114.314 kb on - strand, within ECOLIN_RS20445

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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4,111,740 + -0.3
4,111,740 + +0.6
4,111,753 + -0.1
4,111,753 + +0.5
4,111,753 + +0.7
4,112,114 - ECOLIN_RS20435 0.27 +0.9
4,112,177 - ECOLIN_RS20435 0.40 +0.2
4,112,186 - ECOLIN_RS20435 0.42 -0.1
4,112,320 + ECOLIN_RS20435 0.70 +0.3
4,112,479 - -3.6
4,112,616 + +0.6
4,112,616 + +0.1
4,112,616 + -0.0
4,112,949 - ECOLIN_RS20440 0.45 +0.9
4,112,968 + ECOLIN_RS20440 0.48 -1.4
4,112,970 + ECOLIN_RS20440 0.48 -0.4
4,112,973 - ECOLIN_RS20440 0.48 -0.1
4,112,973 - ECOLIN_RS20440 0.48 +0.1
4,112,973 - ECOLIN_RS20440 0.48 +0.2
4,113,012 + ECOLIN_RS20440 0.53 -0.2
4,113,013 - ECOLIN_RS20440 0.53 +0.0
4,113,013 - ECOLIN_RS20440 0.53 -0.2
4,113,055 - ECOLIN_RS20440 0.58 +0.4
4,113,058 - ECOLIN_RS20440 0.58 +0.2
4,113,150 + ECOLIN_RS20440 0.69 -0.0
4,113,151 - ECOLIN_RS20440 0.69 -0.2
4,113,635 - -0.5
4,113,635 - -0.2
4,113,978 + ECOLIN_RS20445 0.37 +0.1
4,114,005 + ECOLIN_RS20445 0.39 +0.2
4,114,006 - ECOLIN_RS20445 0.39 -0.0
4,114,159 + ECOLIN_RS20445 0.55 +0.5
4,114,160 - ECOLIN_RS20445 0.55 +0.1
4,114,195 + ECOLIN_RS20445 0.58 +0.5
4,114,314 - ECOLIN_RS20445 0.70 -0.2

Or see this region's nucleotide sequence