Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16160

Experiment: Bas21

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16150 and ECOLIN_RS16155 are separated by 163 nucleotidesECOLIN_RS16155 and ECOLIN_RS16160 are separated by 44 nucleotidesECOLIN_RS16160 and ECOLIN_RS16165 are separated by 165 nucleotides ECOLIN_RS16150: ECOLIN_RS16150 - hemolysin III family protein, at 3,274,974 to 3,275,633 _RS16150 ECOLIN_RS16155: ECOLIN_RS16155 - N(4)-acetylcytidine aminohydrolase, at 3,275,797 to 3,276,108 _RS16155 ECOLIN_RS16160: ECOLIN_RS16160 - 6-phospho-beta-glucosidase BglA, at 3,276,153 to 3,277,586 _RS16160 ECOLIN_RS16165: ECOLIN_RS16165 - aminomethyl-transferring glycine dehydrogenase, at 3,277,752 to 3,280,625 _RS16165 Position (kb) 3276 3277 3278Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3275.170 kb on - strand, within ECOLIN_RS16150at 3275.196 kb on + strand, within ECOLIN_RS16150at 3275.197 kb on - strand, within ECOLIN_RS16150at 3275.280 kb on - strand, within ECOLIN_RS16150at 3275.453 kb on + strand, within ECOLIN_RS16150at 3275.473 kb on + strand, within ECOLIN_RS16150at 3275.654 kb on - strandat 3275.666 kb on - strandat 3275.666 kb on - strandat 3275.666 kb on - strandat 3275.666 kb on - strandat 3275.666 kb on - strandat 3275.666 kb on - strandat 3275.822 kb on + strandat 3276.049 kb on - strand, within ECOLIN_RS16155at 3276.114 kb on + strandat 3276.382 kb on + strand, within ECOLIN_RS16160at 3276.516 kb on + strand, within ECOLIN_RS16160at 3276.666 kb on + strand, within ECOLIN_RS16160at 3276.689 kb on + strand, within ECOLIN_RS16160at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.781 kb on - strand, within ECOLIN_RS16160at 3276.866 kb on + strand, within ECOLIN_RS16160at 3276.866 kb on + strand, within ECOLIN_RS16160at 3276.894 kb on + strand, within ECOLIN_RS16160at 3276.894 kb on + strand, within ECOLIN_RS16160at 3276.990 kb on + strand, within ECOLIN_RS16160at 3276.990 kb on + strand, within ECOLIN_RS16160at 3277.022 kb on + strand, within ECOLIN_RS16160at 3277.054 kb on + strand, within ECOLIN_RS16160at 3277.096 kb on - strand, within ECOLIN_RS16160at 3277.242 kb on + strand, within ECOLIN_RS16160at 3277.478 kb on + strandat 3277.482 kb on + strandat 3277.541 kb on + strandat 3277.699 kb on + strandat 3277.731 kb on + strandat 3277.732 kb on - strandat 3278.254 kb on + strand, within ECOLIN_RS16165at 3278.254 kb on + strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.343 kb on - strand, within ECOLIN_RS16165

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas21
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3,275,170 - ECOLIN_RS16150 0.30 +0.8
3,275,196 + ECOLIN_RS16150 0.34 -4.0
3,275,197 - ECOLIN_RS16150 0.34 +0.1
3,275,280 - ECOLIN_RS16150 0.46 -0.2
3,275,453 + ECOLIN_RS16150 0.73 -0.5
3,275,473 + ECOLIN_RS16150 0.76 -0.7
3,275,654 - -0.3
3,275,666 - -2.1
3,275,666 - -3.2
3,275,666 - +1.0
3,275,666 - -0.6
3,275,666 - +0.8
3,275,666 - -0.3
3,275,822 + -0.4
3,276,049 - ECOLIN_RS16155 0.81 +0.5
3,276,114 + +0.7
3,276,382 + ECOLIN_RS16160 0.16 +2.7
3,276,516 + ECOLIN_RS16160 0.25 -1.2
3,276,666 + ECOLIN_RS16160 0.36 -0.6
3,276,689 + ECOLIN_RS16160 0.37 +0.7
3,276,780 + ECOLIN_RS16160 0.44 -1.6
3,276,780 + ECOLIN_RS16160 0.44 -0.1
3,276,780 + ECOLIN_RS16160 0.44 -0.3
3,276,781 - ECOLIN_RS16160 0.44 +0.4
3,276,866 + ECOLIN_RS16160 0.50 -0.2
3,276,866 + ECOLIN_RS16160 0.50 +0.1
3,276,894 + ECOLIN_RS16160 0.52 +0.1
3,276,894 + ECOLIN_RS16160 0.52 +0.1
3,276,990 + ECOLIN_RS16160 0.58 +0.1
3,276,990 + ECOLIN_RS16160 0.58 -1.0
3,277,022 + ECOLIN_RS16160 0.61 -0.4
3,277,054 + ECOLIN_RS16160 0.63 +0.2
3,277,096 - ECOLIN_RS16160 0.66 -2.1
3,277,242 + ECOLIN_RS16160 0.76 -2.3
3,277,478 + +0.6
3,277,482 + +0.7
3,277,541 + +0.3
3,277,699 + -0.1
3,277,731 + +0.0
3,277,732 - +0.3
3,278,254 + ECOLIN_RS16165 0.17 +1.3
3,278,254 + ECOLIN_RS16165 0.17 +0.7
3,278,255 - ECOLIN_RS16165 0.18 +0.3
3,278,255 - ECOLIN_RS16165 0.18 +1.4
3,278,255 - ECOLIN_RS16165 0.18 +0.0
3,278,255 - ECOLIN_RS16165 0.18 +1.9
3,278,343 - ECOLIN_RS16165 0.21 +1.0

Or see this region's nucleotide sequence